J. Mater. Sci. Technol. ›› 2021, Vol. 63: 18-26.DOI: 10.1016/j.jmst.2019.12.030
• Research Article • Previous Articles Next Articles
Hui Wanga,b, Jiaqiang Liua, Chengtao Wangc, Steve Guofang Shena,d,*(
), Xudong Wanga,*(), Kaili Lina,*()
Received:2019-10-29
Revised:2019-12-16
Accepted:2019-12-26
Published:2021-02-10
Online:2021-02-15
Contact:
Steve Guofang Shen,Xudong Wang,Kaili Lin
About author:lklecnu@aliyun.com (K. Lin).1These two authors contributed equally to this work.
Hui Wang, Jiaqiang Liu, Chengtao Wang, Steve Guofang Shen, Xudong Wang, Kaili Lin. The synergistic effect of 3D-printed microscale roughness surface and nanoscale feature on enhancing osteogenic differentiation and rapid osseointegration[J]. J. Mater. Sci. Technol., 2021, 63: 18-26.
| Gene | Primer sequences (F = forward; R = reverse) |
|---|---|
| ALP | F: 5′-TATGTCTGGAACCGCACTGAAC-3' R: 5′-CACTAGCAAGAAGAAGCCTTTGG-3' |
| OCN | F: 5′-GCCCTGACTGCATTCTGCCTCT-3' R: 5′-TCACCACCTTACTGCCCTCCTG-3' |
| COL-I | F: 5′-GCCTCCCAGAACATCACCTA-3' R: 5′-GCAGGGACTTCTTGAGGTTG-3' |
| OPN | F: 5′-CCAAGCGTGGAAACACACAGCC-3' R: 5′-GGCTTTGGAACTCGCCTGACTG-3' |
| β-actin | F: 5′-GTAAAGACCTCTATGCCAACA-3' R: 5′-GGACTCATCGTACTCCTGCT-3' |
Table 1 Primer sequences used for RT-PCR.
| Gene | Primer sequences (F = forward; R = reverse) |
|---|---|
| ALP | F: 5′-TATGTCTGGAACCGCACTGAAC-3' R: 5′-CACTAGCAAGAAGAAGCCTTTGG-3' |
| OCN | F: 5′-GCCCTGACTGCATTCTGCCTCT-3' R: 5′-TCACCACCTTACTGCCCTCCTG-3' |
| COL-I | F: 5′-GCCTCCCAGAACATCACCTA-3' R: 5′-GCAGGGACTTCTTGAGGTTG-3' |
| OPN | F: 5′-CCAAGCGTGGAAACACACAGCC-3' R: 5′-GGCTTTGGAACTCGCCTGACTG-3' |
| β-actin | F: 5′-GTAAAGACCTCTATGCCAACA-3' R: 5′-GGACTCATCGTACTCCTGCT-3' |
Fig. 1. SEM images of control (A, E), nano-control (B, F), micro-3D (C, G) and micro/nano-3D (D, H) groups at low (A, B, C, D) and high (E, F, G, H) magnifications.
Fig. 2. The 3D morphology (A-D) and quantitative analysis of the roughness (E, F, G) of groups control (A), nano-control (B), micro-3D (C) and micro/nano-3D (D). *p < 0.05 compared with control group, ap < 0.05 compared with micro/nano-3D group.
Fig. 3. XRD patterns of the control, nano-control, micro-3D and micro/nano-3D groups.
Fig. 4. Water contact angle of the control, nano-control, micro-3D and micro/nano-3D groups. *p < 0.05 compared with control group, ap < 0.05 compared with micro/nano-3D group.
Fig. 5. SEM images of control (A, E), nano-control (B, F), micro-3D (C, G), micro/nano-3D (D, H) groups at low (E, F, G, H) and high (A, B, C, D) magnifications; XRD patterns of the samples after soaking in SBF for 3 days (I).
Fig. 6. SEM images of adherent BMSCs on the control (A), nano-control (B), micro-3D (C) and micro/nano-3D (D) groups after culturing for 24 h.
Fig. 7. Quantitative analysis of ALP activity of the BMSCs cultured on control, nano-control, micro-3D and micro/nano-3D groups for 4 and 7 days. *p < 0.05 compared with control group, ap < 0.05 compared with micro/nano-3D group.
Fig. 8. RT-PCR analysis of osteogenesis-related gene expressions of BMSCs cultured on the control, nano-control, micro-3D and micro/nano-3D group surfaces for 7 days. *p < 0.05 compared with control group, ap < 0.05 compared with micro/nano-3D group.
Fig. 9. The Alizarin red staining and quantification of extracellular matrix mineralization of BMSCs on the control (A), nano-control (B), micro-3D (C) and micro/nano-3D (D) surfaces after culturing for 21 days. *p < 0.05 compared with control group, ap < 0.05 compared with micro/nano-3D group.
Fig. 10. Histological section images of the osseointegration performance in vivo of the control (A), nano-control (B), micro-3D (C) and micro/nano-3D (D) groups at week 4, scale bar = 200 μm, and the histomorphometry analysis of BIC percentage of the four implants at week 4 (E). *p < 0.05 compared with control group, ap < 0.05 compared with micro/nano-3D group.
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