J. Mater. Sci. Technol. ›› 2020, Vol. 41 ›› Issue (0): 56-67.DOI: 10.1016/j.jmst.2019.09.016

• Research Article • Previous Articles     Next Articles

In vitro insights into the role of copper ions released from selective laser melted CoCrW-xCu alloys in the potential attenuation of inflammation and osteoclastogenesis

Yanjin Luab, Xiongcheng Xuc, Chunguang Yangd**(), Ling Rend, Kai Luoc**(), Ke Yangd, Jinxin Linab*()   

  1. aKey Laboratory of Optoelectronic Materials Chemistry and Physics, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350001, China
    bUniversity of Chinese Academy of Sciences, Beijing 100049, China
    cKey Laboratory of Stomatology, Fujian Medical University, Fuzhou 350001, China
    dInstitute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, China
  • Received:2019-06-03 Revised:2019-08-18 Accepted:2019-09-02 Online:2020-03-15 Published:2020-04-10
  • Contact: Yang Chunguang,Luo Kai,Lin Jinxin
  • About author:

    1The authors contributed equally to this work.

Abstract:

In this study, the CoCrW-xCu alloys (x = 2, 3 and 4 wt%) were fabricated using selective laser melting (abbreviated as Co-2Cu, Co-3Cu and Co-4Cu) with the purpose of reducing the inflammation responses and the activity of osteoclast. The metal ions releasing test showed that when the Cu content was less than 4 wt%, the releasing amount of Co and Cr ions was very small; however, when 4 wt% Cu was added in the CoCrW based alloy, the quantity of Co ions was significantly elevated with respect to the other groups due to the segregation of precipitates in the matrix; the Cu2+ ion quantity of the Co-2Cu, Co-3Cu and Co-4Cu alloys were 0.05, 0.09 and 0.27 μg/(L cm2) after 7 d immersion, respectively; the RT-qPCR and ELISA data indicated that the expression levels of the pro-inflammatory cytokines (TNF-α and IL-6) were down-regulated in the Co-3Cu and Co-4Cu groups, whereas the expression level of the anti-inflammatory cytokine (IL-10) was up-regulated in all CoCrW-xCu alloys; meanwhile, the Cu-containing CoCrW alloys significantly down-regulated the expression of the NF-κB signal pathway in a Cu content-dependent manner, and the downstream transcription factors of NF-κB signal pathway including NFATc1, TRAP and Cath-K were also down-regulated via potentially manipulating the NF-κB signal pathway. After comprehensive consideration, it is considered that the Co-3Cu alloy is a potential material for self-alleviating inflammatory responses.

Key words: Osteoclast, Selective laser melting, Macrophage, Cu-bearing biomedical alloy, Biocompatibility