Cytocompatibility of High Nitrogen Nickel-Free Stainless Steel for Orthopedic Implants

J Mater Sci Technol ›› 2012, Vol. 28 ›› Issue (7): 647-653.

• Iron and Steel • Previous Articles Next Articles

Cytocompatibility of High Nitrogen Nickel-Free Stainless Steel for Orthopedic Implants

Tianchi Ma^1,4), Peng Wan²⁾, Yuyan Cui³⁾, Guirong Zhang⁴⁾, Jiqiang Li⁴⁾, Jihui Liu⁴⁾, Yibin Ren²⁾, Ke Yang²⁾, Li Lu¹⁾

1) School of Stomatology, China Medical University, Shenyang 110002, China
2) Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, China
3) Shenyang the Fourth Hospital of People, Shenyang 110032, China
4) Shenyang Stomatoiogy Hospital, Shenyang 110002, China

Received:2011-07-04 Revised:2011-09-22 Online:2012-07-28 Published:2012-07-28
Contact: Li Lu
Supported by:
the National Natural Science Foundation of China (No. 31000428) and the National Basic Research Program of China (\973 Program", No. 2012CB619101)

Abstract

Abstract: Cytocompatibility of high nitrogen nickel-free stainless steel (HNS) with different nitrogen content was evaluated and compared with a conventional austenitic stainless steel 317L. The MTT assay (3-(4,5-dimethylthiazol2-yl)-2,5-diphenyltetrazolium bromide) was performed on MG63 osteoblasts to assess the cytotoxicity. The expression of selected marker typical of differentiated osteoblasts, such as alkaline phosphatase activity (AKP), was also monitored in MG63 cells cultured on the tested materials. As a result, HNS had higher cell growth than 317L; meanwhile the cytocompatibility was increased with increasing nitrogen content. Furthermore, HNS enhanced osteoblasts differentiation, as confirmed by AKP activity. Overall these facts indicated that HNS had higher cytocompatibility than 317L and the nitrogen content contributed to the higher cytocompatibility of HNS. The influence of nitrogen on surface energy further explained the cytocompatibility of HNS.

Key words: High nitrogen stainless steel, Cytoxicity evaluation, Osteoblasts, Bone, Surface energy

Tianchi Ma, Peng Wan, Yuyan Cui, Guirong Zhang, Jiqiang Li, Jihui Liu, Yibin, Ke Yang, Li Lu. Cytocompatibility of High Nitrogen Nickel-Free Stainless Steel for Orthopedic Implants[J]. J Mater Sci Technol, 2012, 28(7): 647-653.

References

[1 ] D. Williams, R.W. Cahn and M.B. Bever: Concise Encyclopedia of Medical & Dental Materials, Pergamon Press, 1990, 4.

[2 ] L. Kanerva and L. Forstrom: Contact Dermatitis, 2001, 44, 103.

[3 ] S. Torgerser, O.T. Gilhuus-Moe and N.R. Gjerdet: Int. J. Oral Maxillofac. Surg., 1993, 22, 246.

[4 ] G. Stein and J. Menzel: Int. J. Mater. Prod. Technol.-Switzerland, 1995, 10, 290.

[5 ] L. Montanaro, M. Cervellati, D. Campoccia and C.R. Arciola: J. Mater. Sci.-Mater. Med., 2006, 17, 267.

[6 ] A. Yamamoto, Y. Kohyama, D. Kuroda and T. Hanawa: Mater. Sci. Eng. C, 2004, 24, 737.

[7 ] Y.B. Ren, K. Yang and B.C. Zhang: Mater. Lett., 2005, 59, 1785.

[8 ] L. Montanaro, M. Cervellati, D. Campoccia, C. Prati, L. Breschi and C.R. Arciola: Int. J. Artif. Organs, 2005, 28(1), 58.

[9 ] M. Fini, N.N. Aldini, P. Torricelli, G. Glavaresi, V. Borsari, H. Lenger, J. Bernauer, R. Glardino, R. Chiesa and A. Cigada: Biomaterials, 2003, 24(27), 4929.

[10] M. Fini, G. Giavaresi, R. Giardino, H. Lenger, J. Bernauer, L. Rimondini, P. Torricelli, V. Borsari, L. Chiusoli, R. Chiesa and A. Cigada: J. Biomed. Mater. Res. Part B-Appl. Biomater., 2004, 71, 30.

[11] V.G. Gavriljuk and H. Berns: High Nitrogen Steels, Springer-Verlag, Berlin, 1999, 1.

[12] S.J. Kim, T.H. Lee and C.S. Oh: ICASS 2006, Gyeongju, Korea, 22{24 August 2006, eds H.C. Lee, W.Y. Choo, K. Nagai and H. Dong, Korean Institute of Metals and Materials, Seoul, 2006, 594.

[13] P. Wan, Y.B. Ren, B.C. Zhang and K. Yang: Mater. Sci. Eng. C, 2010, 30, 1183.

[14] M. Morra and C. Cassinelli: J. Biomat. Sci.-Polym. E, 1997, 9, 55.

[15] L. Hao and J. Lawrence: J. Phys. D-Appl. Phys., 2003, 36, 1292.

[16] L. Hao and J. Lawrence: Colloid. Surf. B-Biointerfaces, 2004, 34(2), 87.

[17] L. Hao and J. Lawrence: Mater. Sci. Eng. C, 2003, 23, 627.

[18] I. Costa, S.O. Rogero, O.V. Correa, C.T. Kunioshi and M. Saiki: Mater. Sci. Forum, 2005, 498{499, 86.

[19] T. Shi, T.Z. Qin and D.S. Zhan: Oral Biomed., 2010, 1(3), 143. (in Chinese)

[20] G. HochÄortler, J. Bernauer and K. Kriszt: Materials for Medical Engineering, Wiley-VCH, Weinheim, 2000, 204.

[21] E. Borenfrund and J.A. Puerner: Toxicol. Lett., 1985, 24, 119.

[22] J.B. Lian and G.S. Stein: J. Oral Implant., 1993, 19, 95.

[23] J. Ryhanen, M. Kallioinen, W. Serlo, P. Peramaki, J. Junila, P. Sandbik, E. Niemela and J. Tuukkanen: J. Biomed. Mater. Res., 1999, 47, 472.

[24] J.B. Park and R.S. Lakes: Biomaterials: an Introduction, Plenum Press, New York, 1992.

[25] Z. Schwartz, C.H. Lohmann, J. Oefinger, L.F. Bonewald, D.D. Dean and B.D. Boyan: Adv. Dent. Res., 1998, 13, 38.

[26] B.D. Boyan, V.L. Sylvia, Y. Liu, R. Sagun, D.L. Cochran, C.H. Lohmann, D.D. Dean and Z. Schartz: Biomaterials, 1999, 20, 2305.

[27] Z. Schwartz, C.H. Lohmann, A.K. Vocke, V.L. Sylvia, D.L. Cochran, D.D. Dean and B.D. Boyan: J. Biomed. Mater. Res., 2001, 56, 417.

[28] N.J. Hallab, K.J. Bundy, K. O0Connor, R.L. Moses and J.J. Jacobs: Tissue Eng., 2001, 7, 55.

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Cytocompatibility of High Nitrogen Nickel-Free Stainless Steel for Orthopedic Implants

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