J. Mater. Sci. Technol. ›› 2015, Vol. 31 ›› Issue (2): 182-190.DOI: 10.1016/j.jmst.2014.08.007

• Orginal Article • Previous Articles     Next Articles

Promoting Bone Mesenchymal Stem Cells and Inhibiting Bacterial Adhesion of Acid-Etched Nanostructured Titanium by Ultraviolet Functionalization

Guobo Lan1, 2, Mei Li2, Ying Tan3, Lihua Li2, Xiaoming Yang1, Limin Ma2, Qingshui Yin2, Hong Xia2, Yu Zhang2, Guoxin Tan3, Chengyun Ning4   

  1. 1 Southern Medical University, Guangzhou 510515, China; 2 Hospital of Orthopedics, General Hospital of Guangzhou Military Command, Guangzhou 510010, China; 3 Faculty of Light and Chemical, Guangdong University of Technology, Guangzhou 510006, China; 4 College of Materials Science and Technology, South China University of Technology, Guangzhou 510641, China
  • Received:2014-03-05 Online:2015-02-20 Published:2015-07-23
  • Contact: Corresponding author. Prof., Ph.D.; Tel.: +86 13602744495; Fax: +86 20 36654440.E-mail address: luck_2001@126.com (Y. Zhang).
  • Supported by:
    This work was supported by the National Key Basic Research Program of China (Grant No. 2012CB619106), the National Natural Science Foundation of China (Grant No. 81271957), the Military Medical Research “12th Five-Year Plan” General Program of China (Grant No. cws11c268) and Guangdong Provincial Science and Technology Project, China (Grant No. 2012A030400064).

Abstract: Titanium (Ti) and its alloys are used extensively in orthopedic implants because of their excellent biocompatibility, mechanical properties and corrosion resistance. However, titanium-based implant materials face many severe complications, such as implant loosening due to poor osseointegration and bacterial infections, which may lead to implant failure. Hence, preparing a biomaterial surface, which enhances the interactions with host cells and inhibits bacterial adhesion, may be an optimal strategy to reduce the incidence of implant failure. This study aims to improve osseointegration and confer antibacterial properties on Ti through a combination of two surface modifications including nanostructuring generated by acid etching and ultraviolet (UV) light treatment. Our results showed that without UV treatment, the acid etching treatment of Ti surface was effective at both improving the adhesion of bone mesenchymal stem cells (BMSCs) and increasing bacterial adhesion. A further UV treatment of the acid-etched surface however, not only significantly improved the cell adhesion but also inhibited bacterial adhesion. The acid-etched nanostructured titanium with UV treatment also showed a significant enhancement on cell proliferation, alkaline phosphatase (ALP) activity and mineralization. These results suggest that such nanostructured materials with UV treatment can be expected to have a good potential in orthopedic applications.

Key words: UV treatment, Nanostructures, Bone mesenchymal stem cells, Antibacterial activity, Titanium