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J Mater Sci Technol  2008, Vol. 24 Issue (02): 192-196    DOI:
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Antibacterial Characterization of Silver Nanoparticles against E. Coli ATCC-15224
M.Raffi, F.Hussain, T.M.Bhatti, J.I.Akhter, A.Hameed, M.M.Hasan
Department of Chemical and Materials Engineering, Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad-45650, Pakistan
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Abstract  Silver nanoparticles of mean size 16 nm were synthesized by inert gas condensation (IGC) method. Crystalline structure, morphology and nanoparticles size estimation were conducted by X-ray diffraction (XRD) and transmission electron microscopy (TEM). Antibacterial activity of these silver nanoparticles as a function of particles concentration against gram-negative bacterium Escherichia coli (E.coli) was carried out in liquid as well as solid growth media. Scanning electron microscopy (SEM) and TEM studies showed that silver nanoparticles after interaction with E.coli have adhered to and penetrated into the bacterial cells. Antibacterial properties of silver nanoparticles are attributed to their total surface area, as a larger surface to volume ratio of nanoparticles provides more efficient means for enhanced antibacterial activity.
Key words:  Silver      Nanoparticles      Antibacterial      Electron microscopy      
Received:  16 May 2007     
Corresponding Authors:  M. Raffi     E-mail:

Cite this article: 

M.Raffi, F.Hussain, T.M.Bhatti, J.I.Akhter, A.Hameed, M.M.Hasan. Antibacterial Characterization of Silver Nanoparticles against E. Coli ATCC-15224. J Mater Sci Technol, 2008, 24(02): 192-196.

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