Antibacterial Characterization of Silver Nanoparticles against E. Coli ATCC-15224

Abstract

Abstract: Silver nanoparticles of mean size 16 nm were synthesized by inert gas condensation (IGC) method. Crystalline structure, morphology and nanoparticles size estimation were conducted by X-ray diffraction (XRD) and transmission electron microscopy (TEM). Antibacterial activity of these silver nanoparticles as a function of particles concentration against gram-negative bacterium Escherichia coli (E.coli) was carried out in liquid as well as solid growth media. Scanning electron microscopy (SEM) and TEM studies showed that silver nanoparticles after interaction with E.coli have adhered to and penetrated into the bacterial cells. Antibacterial properties of silver nanoparticles are attributed to their total surface area, as a larger surface to volume ratio of nanoparticles provides more efficient means for enhanced antibacterial activity.

Key words: Silver, Nanoparticles, Antibacterial, Electron microscopy

M.Raffi, F.Hussain, T.M.Bhatti, J.I.Akhter, A.Hameed, M.M.Hasan. Antibacterial Characterization of Silver Nanoparticles against E. Coli ATCC-15224[J]. J Mater Sci Technol, 2008, 24(02): 192-196.

References

[1]R.W.Sigel:Mater.Sci.Eng.B,1993,19,37.
[2]C.Suryanarayana:Int.Mater.Rev.,1995,40(2),41.
[3]H.Gleiter:Acta Mater.,2000,48(1),1.
[4]H.J.Lee,S.Y.Yeo and S.H.Jeong:J.Mater.Sci.,2003, 38(10),2199.
[5]I.Sondi and B.Salopek-Sondi:J.Colloid Iuterf.Sci., 2004,275(1),177.
[6]J.R.Morones,J.L.Elechiguerra,A.Camacho,K.Holt, J.B.Kouri,J.T.Ramirez and M.J.Yacaman:Nanotech- nology,2005,16(10),2346.
[7]P.K.Stoimenov,R.L.Klinger,G.L.Marchin and K.J.Klabunde:Langmuir,2002,18,6679.
[8]S.H.Jeong,S.Y.Yeo and S.C.Yi:Mater.Sci.,2005,40, 5407.
[9]S.L.Percivala,P.G.Bowler and D.Russell:J.Hosp.In- fect.,2005,60(1),1.
[10]P.L.Taylor,A.L.Ussher and R.E.Burrelh Biomateri- als,2005,26(35),7221.
[11]A.Panacek,L.Kvitek,R.Prucek,M.Kolar,R.Vecerova, N.Pizurova,V.K.Sharma,T.Nevecna and R.Zborih J. Phys.Chem.B,2006,110(33),16248.
[12]V.Alt,T.Bechert,P.Steinrucke,M.Wagener,P.Seidel, E.Dingeldein,E.Domann and R.Schnettler:Biomate- rials,2{)04,25(18),4383.
[13]J.R.Morones,J.L.Elechiguerra,A.Camacho,K.Holt, J.B.Kouri,.I.T.Ramirez and M.J.Yacaman:Nanotech- nology,2005,16(10),2346.
[14]C.N.Lok,C.M.Ho,R.Chen,Q.Y.He,W.Y.Yu, H.Z.Sun,P.K.H.Tam,J.F.Chiu and C.M.Che:J.Pro- teome Res.,2006,5,916.
[15]A.Oloffs,C.Grossesiestrup,S.Bisson,M.Rinck, R.Rudolph and U.Gross:Biomaterials,1994,15(10), 753.
[16]P.Li,J.Li,C.Z.Wu,Q.S.Wu and J.Li:Nanotechnology, 2005,16(9),1912.
[17]S.T.Zhang,R.W.Fu,D.C.Wu,W.Xu,Q.W.Ye and Z.L.Chen:Carbon,2004,42(15),3209.
[18]C.Baker,A.Pradhan,L.Pakstis,D.J.Pochan and S.I.Shah:J.Nanosci.Nanotechnol,2005,5(2),244.
[19]B.D.Cullity:Elements of X-ray Diffraction,2nd edn, Edison-Wesley Publishing Company Inc,USA,1978.
[20]R.Birringer:Mater.Sci.Eng.A,1989,117,33.
[21]C.G.Granqvisit and R.A.Buharman:J.Appl.Phys., 1976,47(5),2200.
[22]F.E.Kruis,H.Fissan and A.Peled:J Aerosol Sci., 1998,29(5-6),511.
[23]L.B.Kiss,J.Soderlund,G.A.Niklasson and C.G.Granqvist:Nanostruct.Mater.,1999,12(1-4), 327.
[24]M.Raffi,J.I.Akhter and M.M.Hasan:Mater.Chem. Phys.,2006,99(2-3),405.
[25]M.Kokkoris,C.C.Trapalis,S.Kossionides,R.Vlastou, B.Nsouli,R.Grotzschel,S.Spartalis,G.Kordas and T.Paradellis:Nucl.Instrum.Meth.B,2002,188, 67.
[26]K.B.Holt and A.J.Bard:Biochemistry,2005,44(39), 13214.
[27]N.A.Amro,L.P.Kotra,K.Wadu-Mesthrige, A.Bulychev,S.Mobashery and G.Y.Liu:Langmuir, 2000,16,2789.
[28]J.A.Spadaro,T.J.Berger,S.D.Barranco,S.E.Chapin and R.O.Becker:Antimicrob.Agents Chemoth.,1974, 6(5),637.