One-step preparation of porous aminated-silica nanoparticles and their antibacterial drug delivery applications

doi:10.1016/j.jmst.2019.12.015

Abstract

Abstract:

Porous functionalized silica nanoparticles have attracted the interest of researchers as they are excellent carriers for antibacterial drug delivery applications. In this work, porous aminated-silica nanoparticles (SiO₂-NH₂ NPs) were prepared via one-step approach through the ammonia-catalyzed hydrolysis of tetraethylorthosilicate (TEOS) and (3-aminopropyl) triethoxysilane (APTES) in a mixed water-ethanol system. The obtained SiO₂-NH₂ NPs displayed a spherical morphology and relatively uniform size distribution, while the morphology and structure of SiO₂-NH₂ NPs were mainly determined by the order of the reagents added and the pH value of the solution. After characterization, the results showed that there were a large number of -NH₂ groups on the surface of porous SiO₂-NH₂ NPs and that the porous SiO₂-NH₂ NPs had a large surface area of 476 m2 g ^-1 with an average pore width of 4.3 nm. Through an absorbing-releasing experiment and bacterial test, those SiO₂-NH₂ NPs were found to exhibit efficient absorption and release of drugs as well as a pH-dependent release pattern of epirubicin-loaded SiO₂-NH₂ NPs. Meanwhile, SiO₂-NH₂@capsaicin NPs exhibited antibacterial properties. Those porous SiO₂-NH₂ NPs could be a candidate for drug delivery for antibacterial applications owing to their tailored porous structure and high surface area.

Key words: Porous aminated-silica nanoparticles, Surface area, Drug delivery, Antibacterial property

Yongren Wu, Shun Chen, Yang Liu, Zhiwei Lu, Shaokun Song, Yang Zhang, Chuanxi Xiong, Lijie Dong. One-step preparation of porous aminated-silica nanoparticles and their antibacterial drug delivery applications[J]. J. Mater. Sci. Technol., 2020, 50: 139-146.

Figures/Tables 10

Scheme 1 Schematic of the synthesis of porous SiO2-NH2 nanoparticles (NPs) (a) and capsaicin absorption-release abilities (b).

Fig. 1. TEM images of FL-SiO2-NH2 NPs obtained by the traditional order (first tetraethylorthosilicate then (3-aminopropyl) triethoxysilane) of reagents addition (a), MD-SiO2-NH2 NPs obtained by the control order (tetraethylorthosilicate and (3-aminopropyl) triethoxysilane at the same time) for 4?h (b), SiO2-NH2 NPs obtained for 12?h (c) and individual SiO2-NH2 NPs (d).

Fig. 2. Fourier transforms infrared (FT-IR) spectrum (a) and thermogravimetric analysis (TGA) curve of SiO2-NH2 NPs (b).

Fig. 3. Zeta potential of SiO2-NH2 NPs (a), protonated SiO2-NH2 NPs (b) at pH?=?7 in the aqueous phase and the hydrodynamic particle sizes (c).

Fig. 4. Small-angle XRD patterns of SiO2-NH2 NPs (a), nitrogen adsorption-desorption isotherm of SiO2-NH2 NPs (b) and pore size distribution of SiO2-NH2 NPs (c).

Fig. 5. Porous SiO2-NH2 NPs dispersed in water before and after three months.

Fig. 6. Released epirubicin (EPB) percentage from SiO2-NH2@EPB NPs at different pH values (3.6, 4.4, 5.4, 6.6, and 7.4). Each independent experiment was tested three times.

Fig. 7. Photo-luminescent (PL) intensity of capsaicin residue in ethanol after SiO2-NH2 NPs were immersed in capsaicin-ethanol solution for different absorption times (a) and capsaicin release in water for different releasing times (b). Each independent experiment was tested three times.

Fig. 8. The numbers of E. coli after 0.5, 1, 2, 4, 8, 16, and 20?h of E. coli in Luria-Bertani (LB) medium, with 0.5?μg?mL-1 SiO2-NH2 NPs, 0.1?μg?mL-1 SiO2-NH2@capsaicin NPs, and 0.5?μg?mL-1 SiO2-NH2@capsaicin NPs added to E. coli in Luria-Bertani (LB) medium. Each independent experiment was tested three times.

Fig. 9. Antibacterial activity of SiO2-NH2@capsaicin NPs at (a) 8?h, (b) 12?h and (c) 24?h against E. coli (insert: part 3, after the paper was removed). SiO2-NH2 NPs, 0.1?μg?mL-1 SiO2-NH2 @capsaicin NPs, and 0.5?μg?mL-1 SiO2-NH2@capsaicin NPs were added to parts 1 to 3, respectively.

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