Please wait a minute...
J Mater Sci Technol  2008, Vol. 24 Issue (02): 192-196     DOI:
Research Articles Current Issue | Archive | Adv Search |
Antibacterial Characterization of Silver Nanoparticles against E. Coli ATCC-15224
M.Raffi, F.Hussain, T.M.Bhatti, J.I.Akhter, A.Hameed, M.M.Hasan
Department of Chemical and Materials Engineering, Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad-45650, Pakistan
Download: PDF (533 KB)   HTML (1 KB) 
Export: BibTeX | EndNote (RIS)      
Abstract  Silver nanoparticles of mean size 16 nm were synthesized by inert gas condensation (IGC) method. Crystalline structure, morphology and nanoparticles size estimation were conducted by X-ray diffraction (XRD) and transmission electron microscopy (TEM). Antibacterial activity of these silver nanoparticles as a function of particles concentration against gram-negative bacterium Escherichia coli (E.coli) was carried out in liquid as well as solid growth media. Scanning electron microscopy (SEM) and TEM studies showed that silver nanoparticles after interaction with E.coli have adhered to and penetrated into the bacterial cells. Antibacterial properties of silver nanoparticles are attributed to their total surface area, as a larger surface to volume ratio of nanoparticles provides more efficient means for enhanced antibacterial activity.
Key wordsSilver      Nanoparticles      Antibacterial      Electron microscopy      
Received: 16 May 2007      Published: 10 October 2009
Corresponding Authors: M. Raffi     E-mail:

Cite this article:

M.Raffi, F.Hussain, T.M.Bhatti, J.I.Akhter, A.Hameed, M.M.Hasan. Antibacterial Characterization of Silver Nanoparticles against E. Coli ATCC-15224. J Mater Sci Technol, 2008, 24(02): 192-196 .

URL:      OR

[3]H.Gleiter:Acta Mater.,2000,48(1),1.
[4]H.J.Lee,S.Y.Yeo and S.H.Jeong:J.Mater.Sci.,2003, 38(10),2199.
[5]I.Sondi and B.Salopek-Sondi:J.Colloid Iuterf.Sci., 2004,275(1),177.
[6]J.R.Morones,J.L.Elechiguerra,A.Camacho,K.Holt, J.B.Kouri,J.T.Ramirez and M.J.Yacaman:Nanotech- nology,2005,16(10),2346.
[7]P.K.Stoimenov,R.L.Klinger,G.L.Marchin and K.J.Klabunde:Langmuir,2002,18,6679.
[8]S.H.Jeong,S.Y.Yeo and S.C.Yi:Mater.Sci.,2005,40, 5407.
[9]S.L.Percivala,P.G.Bowler and D.Russell:J.Hosp.In- fect.,2005,60(1),1.
[10]P.L.Taylor,A.L.Ussher and R.E.Burrelh Biomateri- als,2005,26(35),7221.
[11]A.Panacek,L.Kvitek,R.Prucek,M.Kolar,R.Vecerova, N.Pizurova,V.K.Sharma,T.Nevecna and R.Zborih J. Phys.Chem.B,2006,110(33),16248.
[12]V.Alt,T.Bechert,P.Steinrucke,M.Wagener,P.Seidel, E.Dingeldein,E.Domann and R.Schnettler:Biomate- rials,2{)04,25(18),4383.
[13]J.R.Morones,J.L.Elechiguerra,A.Camacho,K.Holt, J.B.Kouri,.I.T.Ramirez and M.J.Yacaman:Nanotech- nology,2005,16(10),2346.
[14]C.N.Lok,C.M.Ho,R.Chen,Q.Y.He,W.Y.Yu, H.Z.Sun,P.K.H.Tam,J.F.Chiu and C.M.Che:J.Pro- teome Res.,2006,5,916.
[15]A.Oloffs,C.Grossesiestrup,S.Bisson,M.Rinck, R.Rudolph and U.Gross:Biomaterials,1994,15(10), 753.
[16]P.Li,J.Li,C.Z.Wu,Q.S.Wu and J.Li:Nanotechnology, 2005,16(9),1912.
[17]S.T.Zhang,R.W.Fu,D.C.Wu,W.Xu,Q.W.Ye and Z.L.Chen:Carbon,2004,42(15),3209.
[18]C.Baker,A.Pradhan,L.Pakstis,D.J.Pochan and S.I.Shah:J.Nanosci.Nanotechnol,2005,5(2),244.
[19]B.D.Cullity:Elements of X-ray Diffraction,2nd edn, Edison-Wesley Publishing Company Inc,USA,1978.
[21]C.G.Granqvisit and R.A.Buharman:J.Appl.Phys., 1976,47(5),2200.
[22]F.E.Kruis,H.Fissan and A.Peled:J Aerosol Sci., 1998,29(5-6),511.
[23]L.B.Kiss,J.Soderlund,G.A.Niklasson and C.G.Granqvist:Nanostruct.Mater.,1999,12(1-4), 327.
[24]M.Raffi,J.I.Akhter and M.M.Hasan:Mater.Chem. Phys.,2006,99(2-3),405.
[25]M.Kokkoris,C.C.Trapalis,S.Kossionides,R.Vlastou, B.Nsouli,R.Grotzschel,S.Spartalis,G.Kordas and T.Paradellis:Nucl.Instrum.Meth.B,2002,188, 67.
[26]K.B.Holt and A.J.Bard:Biochemistry,2005,44(39), 13214.
[27]N.A.Amro,L.P.Kotra,K.Wadu-Mesthrige, A.Bulychev,S.Mobashery and G.Y.Liu:Langmuir, 2000,16,2789.
[28]J.A.Spadaro,T.J.Berger,S.D.Barranco,S.E.Chapin and R.O.Becker:Antimicrob.Agents Chemoth.,1974, 6(5),637.
[1] Nosheen Zafar, Shahzadi Shamaila, Jawad Nazir, Rehana Sharif, Muhammad Shahid Rafique, Jalees Ul-Hasan, Syeda Ammara, Hina Khalid. Antibacterial Action of Chemically Synthesized and Laser Generated Silver Nanoparticles against Human Pathogenic Bacteria[J]. J. Mater. Sci. Technol., 2016, 32(8): 721-728.
[2] Kun Luo, Yongdong Xiang, Haiming Wang, Li Xiang, Zhihong Luo. Multiple-Sized Amphiphilic Janus Gold Nanoparticles by Ligand Exchange at Toluene/Water Interface[J]. J. Mater. Sci. Technol., 2016, 32(8): 733-737.
[3] Zahra Amirsardari, Rouhollah Mehdinavaz-Aghdam, Masoud Salavati-Niasari, Mohammad Reza Jahannama. Influence of ZrB2 Nanoparticles on the Mechanical and Thermal Behaviors of Carbon Nanotube Reinforced Resol Composite[J]. J. Mater. Sci. Technol., 2016, 32(7): 611-616.
[4] Chandni Khurana, Purnima Sharma, O.P. Pandey, Bhupendra Chudasama. Synergistic Effect of Metal Nanoparticles on the Antimicrobial Activities of Antibiotics against Biorecycling Microbes[J]. J. Mater. Sci. Technol., 2016, 32(6): 524-532.
[5] Li Nan, Guogang Ren, Donghui Wang, Ke Yang. Antibacterial Performance of Cu-Bearing Stainless Steel against Staphylococcus aureus and Pseudomonas aeruginosa in Whole Milk[J]. J. Mater. Sci. Technol., 2016, 32(5): 445-451.
[6] Tingzhi Liu, Yangyang Li, Huan Ke, Yuhai Qian, Shuwang Duo, Yanli Hong, Xinyuan Sun. Chemical Bath Co-deposited ZnS Film Prepared from Different Zinc Salts: ZnSO4-Zn(CH3COO)2, Zn(NO3)2-Zn(CH3COO)2, or ZnSO4-Zn(NO3)2[J]. J. Mater. Sci. Technol., 2016, 32(3): 207-217.
[7] Zhijun Jia, Jun Wang, Yi Wang, Bingyang Li, Baoguo Wang, Tao Qi, Xin Wang. Interfacial Synthesis of δ-MnO2 Nano-sheets with a Large Surface Area and Their Application in Electrochemical Capacitors[J]. J. Mater. Sci. Technol., 2016, 32(2): 147-152.
[8] Wei Xu, Qingsong Xu, Qijin Huang, Ruiqin Tan, Wenfeng Shen, Weijie Song. Fabrication of Flexible Transparent Conductive Films with Silver Nanowire by Vacuum Filtration and PET Mold Transfer[J]. J. Mater. Sci. Technol., 2016, 32(2): 158-161.
[9] Kuang-Hsu Wu, Xue Leng, Ian R. Gentle, Da-Wei Wang. Enhanced Electroactivity of Facet-Controlled Co3O4Nanocrystals for Enzymeless Biosensing[J]. J. Mater. Sci. Technol., 2016, 32(1): 24-27.
[10] Yongguang Wang, Xiangyu Wang, Bo Sun, Shaochun Tang, Xiangkang Meng. Concentration-dependent Morphology Control of Pt-coated-Ag Nanowires and Effects of Bimetallic Interfaces on Catalytic Activity[J]. J. Mater. Sci. Technol., 2016, 32(1): 41-47.
[11] Hefei Huang, Chao Yang, Massey de los Reyes, Yongfeng Zhou, Long Yan, Xingtai Zhou. Effect of Milling Time on the Microstructure and Tensile Properties of Ultrafine Grained Ni-SiC Composites at Room Temperature[J]. J. Mater. Sci. Technol., 2015, 31(9): 923-929.
[12] Qijin Huang, Wenfeng Shen, Ruiqin Tan, Wei Xu, Weijie Song. Separation of Silver Nanocrystals for Surface-enhanced Raman Scattering Using Density Gradient Centrifugation[J]. J. Mater. Sci. Technol., 2015, 31(8): 834-839.
[13] Zheng Ma, Ling Ren, Rui Liu, Ke Yang, Yu Zhang, Zhenhua Liao, Weiqiang Liu, Min Qi, R.D.K. Misra. Effect of Heat Treatment on Cu Distribution, Antibacterial Performance and Cytotoxicity of Ti-6Al-4V-5Cu Alloy[J]. J. Mater. Sci. Technol., 2015, 31(7): 723-732.
[14] S. Snega, K. Ravichandran, M. Baneto, S. Vijayakumar. Simultaneous Enhancement of Transparent and Antibacterial Properties of ZnO Films by Suitable F Doping[J]. J. Mater. Sci. Technol., 2015, 31(7): 759-765.
[15] Hao Yang, Ling Wang, Bing Yuan, Kai Yang, Yuqiang Ma. Adhesion of an Ultrasmall Nanoparticle on a Bilayer Membrane is Still Size and Shape Dependent[J]. J. Mater. Sci. Technol., 2015, 31(6): 660-663.
ISSN: 1005-0302
CN: 21-1315/TG
About JMST
Privacy Statement
Terms & Conditions
Editorial Office: Journal of Materials Science & Technology , 72 Wenhua Rd.,
Shenyang 110016, China
Tel: +86-24-83978208
Copyright © 2016 JMST, All Rights Reserved.