Novel antibacterial cellulose diacetate-based composite 3D scaffold as potential wound dressing

doi:10.1016/j.jmst.2020.12.007

Abstract

Abstract:

In this work, novel antibacterial cellulose diacetate/poly(ethylenimine)/hyaluronic acid (CDA/PEI/HA) composite three-dimension (3D) scaffolds were prepared through electrospinning and post-processing. Firstly, CDA/PEI 2D composite nanofiber membranes were prepared by electrospinning, and then the CDA/PEI/HA 3D composite scaffolds were fabricated by post-processing and freeze-drying of CDA/PEI membranes. In particular, HA was added to improve the biocompatibility of composite scaffolds. Compared with CDA/PEI scaffolds, CDA/PEI/HA composite scaffolds showed higher water absorbing rate, higher water retention rate and higher mechanical strength. Moreover, CDA/PEI/HA composite scaffolds with abundant positive charge were benefit for improving antibacterial activity. CDA/PEI/HA scaffolds with biology function of HA were benefit for improving hemocompatibility and cell proliferation of CDA/PEI scaffolds. In summary, antibacterial CDA/PEI/HA scaffolds could protect wound from bacterial infection, improve cellular behavior and accelerate wound healing. Thus, CDA/PEI/HA scaffolds could be potential application for wound dressing.

Key words: Cellulose diacetate, Electrospinning, 3D scaffold, Wound dressing

Wencheng Liang, Mingli Jiang, Junyong Zhang, Xiaoming Dou, Yan Zhou, Yun Jiang, Li Zhao, Meidong Lang. Novel antibacterial cellulose diacetate-based composite 3D scaffold as potential wound dressing[J]. J. Mater. Sci. Technol., 2021, 89: 225-232.

Figures/Tables 11

Fig. 1. The preparation of composite scaffolds.

Table 1 Compositions of composite scaffolds.

	m_(CDA/PEI) (g)	m_(HA) (g)	H₂O (g)
CHP-1	0.6	0	20
CHP-2	0.575	0.025	20
CHP-3	0.55	0.05	20
CHP-4	0.525	0.075	20
CHP-5	0.5	0.1	20

Fig. 2. FT-IR spectra of different composite scaffolds.

Fig. 3. SEM images of different composite scaffolds.

Fig. 4. Porosity of different composite scaffolds.

Fig. 5. Water absorbing rate of different composite scaffolds.

Fig. 6. Water retention rate of different composite scaffolds within 1 day.

Fig. 7. Tensile strength (a) and elongation (b) of different composite scaffolds.

Fig. 8. (a) Antibacterial activities of different composite scaffolds against E. coli and S. aureus and (b) kill bacterial rate of different composite scaffolds.

Fig. 9. Hemolysis ratio of different composite scaffolds.

Fig. 10. Morphology of cells on the scaffolds surface at 6 days (a), cell attachment rate within 1 day (b) and the cell viability of different composite scaffolds at 2, 4 and 6 days (c). (*p < 0.05, **p < 0.01, indicated that the means of significantly different compared with control group).

References 44

[1]	D. Simoes, S.P. Miguel, M.P. Ribeiro, P. Coutinho, A.G. Mendonca, I.J. Correia, Eur. J. Pharm. Biopharm. 127 (2018) 130-141. DOI URL
[2]	Y. Qin, Y. Liu, L. Yuan, H. Yong, J. Liu, Food Hydrocoll. 96 (2019) 102-111. DOI URL
[3]	C. Lopez-Iglesias, J. Barros, I. Ardao, F.J. Monteiro, C. Alvarez-Lorenzo, J.L.Gomez-Amoza, C.A. Garcia-Gonzalez, Carbohydr. Polym. 204 (2019) 223-231. DOI URL
[4]	X. Ding, S. Duan, X. Ding, R. Liu, F.J. Xu, Adv. Funct. Mater. 28 (2018), 1802140. DOI URL
[5]	S. Wang, H. Zheng, L. Zhou, F. Cheng, Z. Liu, H. Zhang, L. Wang, Q. Zhang, NanoLett. 20 (2020) 5149-5158. DOI URL
[6]	H.J. Zhang, M.X. Peng, T. Cheng, P. Zhao, L.P. Qiu, J. Zhou, G.Z. Lu, J.H. Chen, J.Mater. Sci. 53 (2018) 14944-14952.
[7]	M. Akter, M.T. Sikder, M.M. Rahman, A. Ullah, K.F.B.Hossain, S. Banik, T.Hosokawa, T. Saito, M. Kurasaki, J. Adv. Res. 9 (2018) 1-16. DOI URL
[8]	J.C. Lin, X. Chen, C. Chen, J. Hu, C. Zhou, X. Cai, W. Wang, C. Zheng, P. Zhang, J. Cheng Z. Guo, H. Liu, ACS Appl. Mater. Interfaces 10 (2018) 6124-6136. DOI URL
[9]	J. He, Y. Liang, M. Shi, B. Guo, Chem. Eng. J. 385 (2020), 123464. DOI URL
[10]	W. Cheng, C. Yang, X. Ding, A.C. Engler, J.L. Hedrick, Y.Y. Yang, Biomacromolecules 16 (2015) 1967-1977. DOI PMID
[11]	M. Naseri-Nosar, Z.M. Ziora, Carbohydr. Polym. 189 (2018) 379-398. DOI URL
[12]	M.A. Teixeira, M.C. Paiva, M.T.P. Amorim, H.P. Felgueiras, Nanomaterials Base l10 (2020) 557.
[13]	A. Tchobanian, H. Van Oosterwyck, P. Fardim, Carbohydr. Polym. 205 (2019) 601-625. DOI URL
[14]	K.Y. Lee, L. Jeong, Y.O. Kang, S.J. Lee, W.H. Park, Adv. Drug Deliv. Rev. 61 (2009) 1020-1032. DOI URL
[15]	H. Hanabusa, E.I. Izgorodina, S. Suzuki, Y. Takeoka, M. Rikukawa, M. Yoshizawa-Fujita , Green Chem. 20 (2018) 1412-1422. DOI URL
[16]	P.C. Xu, C.L. Cen, N.N. Chen, H. Lin, Q. Wang, N.F. Xu, J.E. Tang, Z.G. Teng, J.Colloid Interface Sci. 526 (2018) 194-200.
[17]	Y. Wang, L. Zhang, A. Lu, ACS Appl. Mater. Interfaces 11 (2019) 41710-41716. DOI URL
[18]	R. Tong, G. Chen, D. Pan, H. Qi, R. Li, J. Tian, F. Lu, M. He, Biomacromolecules 20 (2019) 2096-2104. DOI URL
[19]	K.W. Kolewe, K.M. Dobosz, K.A. Rieger, C.C. Chang, T. Emrick, J.D. Schiffman, ACS Appl. Mater. Interfaces 8 (2016) 27585-27593. DOI URL
[20]	Y. Chen, A. Mensah, Q. Wang, D. Li, Y. Qiu, Q. Wei, Mater. Sci. Eng. C 115 (2020),111155.
[21]	M.K. Gaydhane, J.S. Kanuganti, C.S. Sharma, , J. Mater. Res. 35 (2020) 600-609. DOI URL
[22]	F. Li, P. Fei, B. Cheng, J. Meng, L. Liao, Carbohydr. Polym. 216 (2019) 312-321. DOI URL
[23]	Y. Yang, W. Li, D.G. Yu, G. Wang, G.R. Williams, Z. Zhang, Carbohydr. Polym. 203 (2019) 228-237. DOI URL
[24]	W. Ma, M. Zhang, Z. Liu, M. Kang, C. Huang, G. Fu, , J. Membr. Sci. 570- 571 (2019) 303-313.
[25]	G. Yang, X. Li, Y. He, J. Ma, G. Ni, S. Zhou, Prog. Polym. Sci. 81 (2018) 80-113. DOI URL
[26]	J. Xie, B. Ma, P.L. Michael, Adv. Healthcare Mater. 1 (2012) 674-678. DOI URL
[27]	J. Lou, R. Stowers, S. Nam, Y. Xia, O. Chaudhuri, Biomaterials 154 (2018) 213-222. DOI URL
[28]	W. Chen, S. Chen, Y. Morsi, H. El-Hamshary, M. El-Newhy, C. Fan, X. Mo, ACSAppl. Mater. Interfaces 8 (2016) 24415-24425.
[29]	S. Tiwari, P. Bahadur, Int. J. Biol. Macromol. 121 (2019) 556-571. DOI URL
[30]	A. Abednejad, A. Ghaee, J. Nourmohammadi, A.A. Mehrizi, Carbohydr. Polym. 222 (2019), 115033. DOI URL
[31]	S. Liu, Q. Zhang, J. Yu, N. Shao, H. Lu, J. Guo, X. Qiu, D. Zhou, Y. Huang, Adv.Healthcare Mater. 9 (2020), 2000198.
[32]	G. Huang, H. Huang, J. Control. Release 278 (2018) 122-126. DOI URL
[33]	Y. Pan, X. Zhao, X. Li, P. Cai, Polymers (Basel) 11 (2019) 1846. DOI URL
[34]	A.S. Montaser, M. Rehan, W.M.El-Senousy, S.Zaghloul,Carbohydr. Polym. 244 (2020), 116479.
[35]	H. Ao, W. Jiang, Y. Nie, C. Zhou, J. Zong, M. Liu, X. Liu, Y. Wan, Polym. Test. 86 (2020), 106490. DOI URL
[36]	Z. Hu, L. Zhang, L. Zhong, Y. Zhou, J. Xue, Y. Li, Carbohydr. Polym. 219 (2019) 290-297. DOI URL
[37]	X. Zhang, Z. Wang, C.Y. Tang, J. Ma, M. Liu, M. Ping, M. Chen, Z. Wu, J. Membr.Sci. 549 (2018) 165-172.
[38]	X. Yang, W. Liu, G. Xi, M. Wang, B. Liang, Y. Shi, Y. Feng, X. Ren, C. Shi, Carbohydr. Polym. 222 (2019), 115012. DOI URL
[39]	Z. Wang, W. Hu, Y. Du, Y. Xiao, X. Wang, S. Zhang, J. Wang, C. Mao, ACS Appl.Mater. Interfaces 12 (2020) 13622-13633.
[40]	W. Liang, J. Hou, X. Fang, F. Bai, T. Zhu, F. Gao, C. Wei, X. Mo, M. Lang, Appl.Surf. Sci. 443 (2018) 374-381.
[41]	H. Lei, C. Zhu, D. Fan, Carbohydr. Polym. 239 (2020), 116249. DOI URL
[42]	K. Wang, J. Wang, L. Li, L. Xu, N. Feng, Y. Wang, X. Fei, J. Tian, Y. Li, Chem. Eng. J. 372 (2019) 216-225. DOI URL
[43]	Y. Liu, J. Jia, T. Gao, X. Wang, J. Yu, D. Wu, F. Li, J. Colloid Interface Sci. 576 (2020) 302-312. DOI URL
[44]	R. Ma, Y. Wang, H. Qi, C. Shi, G. Wei, L. Xiao, Z. Huang, S. Liu, H. Yu, C. Teng, H. Liu V. Murugadoss, J. Zhang, Y. Wang, Z. Guo, Compos. Part B 167 (2019) 396-405. DOI URL