Electrochemical Immunosensor for Detection of Atrazine Based on Polyaniline/Graphene

doi:10.1016/j.jmst.2016.04.004

Abstract

Abstract: In this study, a novel layer-by-layer polyaniline/graphene (PANi/Gr) structure for electrochemical detection of atrazine was developed. Gr film was synthesized by thermal chemical vapor deposition (CVD) method and transferred onto the PANi-predeposited microelectrode. The properties of PANi/Gr film were thoroughly investigated by high-resolution transmission electron microscopy and Raman techniques. The most attractive feature of this system is a suitable microenvironment, which could provide an amplification of the conductive signal, thus may contribute to enhancing electron transfer and subsequently improve the sensitivity in electrochemical measurements. With low detection limit (～ 43?×?10^-12?g/L), acceptable stability and good reproducibility, the proposed electrochemical immunosensor could be advantageously extended for multiplexed detection of other agents of environmental pollution.

Key words: Electrochemical immunosensor, Polyaniline, Graphene, Atrazine

Nguyen Van Chuc, Nguyen Hai Binh, Cao Thi Thanh, Nguyen Van Tu, Nguyen Le Huy, Nguyen Tuan Dzung, Phan Ngoc Minh, Vu Thi Thu, Tran Dai Lam. Electrochemical Immunosensor for Detection of Atrazine Based on Polyaniline/Graphene[J]. J. Mater. Sci. Technol., 2016, 32(6): 539-544.

Figures/Tables 7

Transferring the graphene film from Cu tape onto Pt/PANi working electrode.

Principle of label-free electrochemical detection of ATZ by PANi/Gr immunosensor.

Raman spectra of the films: PANi, Gr, and PANi/Gr.

HR-TEM image of the Gr layer after transferring from Cu substrate to a Cu grid for TEM examination.

Electrochemical behavior of Pt/PANi/Gr before and after GA immobilization.

SWV response of the fabricated immunosensor with ATZ concentration from 10-11 to 10-7?mol/L.

Linear output of the fabricated immunosensor with ATZ concentration from 10-11 to 10-7?mol/L.

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