Marine bacteria inhibit corrosion of steel via synergistic biomineralization

doi:10.1016/j.jmst.2020.03.089

Abstract

Abstract:

Metal corrosion often results in incalculable economic loss and significant safety hazards. Although numerous traditional methods have been used to mitigate the issue, such as coating and corrosion inhibitors, they are environmentally unfriendly and difficult to maintain. Therefore, in this study, an environmental approach was taken to protect steels from corrosion in a multi-species bacterial environment via synergistic biomineralization. The marine bacterium Pseudoalteromonas lipolytica mixed with Bacillus subtilis or Pseudomonas aeruginosa strains offered extraordinary corrosion protection for steel. The surface characterization and electrochemical tests showed that the biomineralized film generated by the mixed bacteria was more compact and protective than that induced by a single bacterium. Herein, we found that the synergistic mechanisms were rather different for the different bacterial groups. For Pseudoalteromonas lipolytica and Bacillus subtilis group, the related mechanisms were due to the increase of pH in the medium, secretion of carbonic anhydrase. As for Pseudoalteromonas lipolytica and Pseudomonas aeruginosa group, the synergistic mechanism was attributed to the inhibiting corrosive bacteria in biofilm by the growth advantage of Pseudoalteromonas lipolytica. Therefore, this study may introduce a new perspective for future use of biomineralization in a real marine environment.

Key words: Bacillus subtilis, Pseudoalteromonas lipolytica, Pseudomonas aeruginosa, Biomineralized film, Anticorrosion, Steel

Na Guo, Yanan Wang, Xinrui Hui, Qianyu Zhao, Zhenshun Zeng, Shuai Pan, Zhangwei Guo, Yansheng Yin, Tao Liu. Marine bacteria inhibit corrosion of steel via synergistic biomineralization[J]. J. Mater. Sci. Technol., 2021, 66: 82-90.

Figures/Tables 11

Fig. 1. SEM images of the steel specimens inoculated with different strains (P. lipolytica, B. subtilis and P. aeruginosa) and control group (sterile marine broth). Image of two different magnitudes are shown in the up (a-d) and down panel (e-h) for 14 days of immersion testing.

Fig. 2. SEM images of the steel specimens inoculated with PB and PP groups. Image of two different magnitudes are shown in the up (a, b) and down panel (c, d) for 14 days of immersion testing.

Fig. 3. Cross-sectional SEM images of product films induced by different groups (control, PB and PP group) on day 14.

Fig. 4. Composition and structure of calcium magnesium carbonate was confirmed by XRD spectrum measured on the steel specimens inoculated with PB and PP groups (red and blue lines), and the corrosion products composition (Fe3O4) was measured in the control group (black line) after 14 days of immersion in marine broth, respectively.

Fig. 5. Nyquist diagrams measured on the steel specimens inoculated with different groups (P. lipolytica, B. subtilis, P. aeruginosa, PB and PP) and control group (sterile marine broth) for 13 days.

Fig. 6. Equivalent circuits for the different groups (a) before the compact biomineralized film formed on the steel (b) after the compact biomineralized film formed on the steel.

Fig. 7. Corrosion rates from a weight loss test (up to 30 days) were performed for different groups (control, B. subtilis, P. lipolytica, P. aeruginosa PB and PP) in marine broth. Data are from three independent tests and one standard deviation is shown.

Fig. 8. Images of the optical profilometry of the pit morphology on the steel surfaces after removing the product films for different group (control, P. lipolytica, B. subtilis, P. aeruginosa, PB and PP).

Fig. 9. Time dependence of (a, b) concentration of dissolved oxygen and (c, d) pH values in the sterile marine broth (control) and the marine broths inoculated with different groups (P. lipolytica, B. subtilis, P. aeruginosa, PB and PP).

Fig. 10. Nyquist diagrams measured on the coupons immersed in medium with (a) B. subtilis (b) P. lipolytica and (c) co-cultures of B. subtilis and P. lipolytica, containing carbonic anhydrase inhibitor (CAI, acetazolamide).

Fig. 11. Percent community abundance on genus level (%) of (a) PB group and (b) PP group in co-cultures medium for 7 days.

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