An in vivo Evaluation of Ultra-fine Grained Titanium Implants

Abstract

Abstract:

The present work focuses mainly on an in vivo evaluation of ultra fine grained titanium (UFG-Ti) obtained by severe plastic deformation (SPD). The SPD on commercially produced Grade 2 titanium (Cp-Ti) resulted in the refinement of the grain size by several orders of magnitude. Polished surfaces having similar roughness from both UFG-Ti and Cp-Ti were prepared. In vitro test revealed the presence of fibronectin, which was involved in the attachment of the cells to the substrate. Phase contrast micrographs showed the highest signal of fibronectin in UFG-Ti, indicating that it is more cytocompatible than Cp-Ti. In vivo tests, by subcutaneous implantation of the metals in the rats showed the better biocompatibility of UFG-Ti over Cp-Ti. The improved biocompatibility of UFG-Ti was attributed to the presence of surface discontinuities (in the form of nano-defects), surface energy, higher wettability, surface stress and stable TiO₂ films, which increased the protein adsorption on the surface.

Key words: Surfaces, Grain size, Titanium, In vivo, Biocompatibility

S. Bindu,K.P. Sanosh,K. Smetana,A. Balakrishnan,T.N. Kim. An in vivo Evaluation of Ultra-fine Grained Titanium Implants[J]. J Mater Sci Technol, 2009, 25(04): 556-560.

References

[1 ] K.S. Kumar, H. Van Swygenhoven and S. Suresh: Acta Mater., 2003, 51, 5743.
[2 ] M.A. Meyers, A. Mishra and D.J. Benson: Prog. Mater. Sci., 2006, 51, 427.
[3 ] R.Z. Valiev, R.K. Islamgaliev and I.V. Alexandrov: Prog. Mater. Sci., 2000, 45, 103.
[4 ] R.Z. Valiev: Nat. Mater., 2004, 3, 511.
[5 ] R.Z. Valiev and T.G. Langdon: Prog. Mater. Sci., 2006, 51, 881.
[6 ] A. Balyanov, J. Kutnyakova, N.A. Amirkhanova, V.V. Stolyarov, R.Z. Valiev and X.Y. Liao: Scripta Mater., 2004, 51, 225.
[7 ] H.J. Rack and J.I. Qazi: Mater. Sci. Eng. C, 2006, 26, 1269.
[8 ] P.L. Snyder and T.J. Webster: Mater. Res. Soc. Symp. Proc., 2007, 950, 13.
[9 ] C.L. Chu, X.Y. Xue, J.C. Zhu and Z. Yin: Mater. Sci. Eng. A, 2006, 429, 18.
[10] Z.D. Cui, M.F. Chen, L.Y. Zhang, R.X. Hu, S.L. Zhu and X.J. Yang: Mater. Sci. Eng. C, 2008, 28, 1117.
[11] M. Jayaraman, U. Meyer, M. BÄuhner, U. Joos and H.P. Wiesmann: Biomaterials, 2004, 25, 625.
[12] S.P. Xavier, P.S.P. Carvalho, M.M. Beloti and A.L. Rosa: J. Dent., 2003, 31, 173.
[13] Y.L. Zhou, M. Niinomi, T. Akahori, H. Fukui and H. Toda: Mater. Sci. Eng. C, 2005, 398, 28.
[14] G.X. Tang, R. J. Zhang, Y.N. Yan, Z.X. Zhu and J. B. Peng: Rare Metal Mat. Eng., 2005, 34, 821. (in Chinese)
[15] H.M. Kim, F. Miyaji and T. Kokubo: J. Biomed. Mater. Res., 1996, 32, 409.
[16] H. Tshizaw and M.Ogimo: J. Biomed. Mater. Res., 1995, 29, 65.
[17] S.H. Chon, T.N. Kim and J.K. Park: Met. Mater. Int., 2004, 10, 567.
[18] A. Balakrishnan, B.C. Lee, T.N. Kim and B.B. Panigrahi: Trends Biomater. Artif. Organs, 2008, 22, 54.
[19] T.N. Kim, A. Balakrishnan, B.C. Lee, W.S. Kim, B. Dvorankova, K. Smetana, J.K. Park and B.B. Panigrahi: J. Mater. Sci.-Mater. M., 2008, 19, 553.
[20] C. Oakley and D. Brunette: J. Cell Sci., 1993, 106, 343.
[21] B.D. Boyan, T.W. Hummert, D.D. Dean and Z. Schwartz: Biomaterials, 1996, 17, 137.
[22] D.D. Deligianni, N.Katsala, S. Ladas, D. Sotiropoulou, J. Amedee and Y.F. Missirlis: Biomaterials, 2001, 22, 1241.
[23] M.C. Advinculaa, F.G. Rahemtullaa, R.C. Advinculac, E.T. Adae, J.E. Lemonsa and S.L. Bellisa: Biomaterials, 2006, 27, 2201.
[24] V. Fronkova, Z. Holkova, F.T. Liu, J. Homolka, D.C. Rijken, S. Andre, N.V. Bovin, K. Smetana Jr and H.J. Gabius: Folia Biol. (Praha), 1999, 45, 157.
[25] K. Smetana Jr: Exp. Mol. Pathol., 1987, 46, 258.
[26] V. Wirtha, C. Comtea, P. Lagneaua, M. Exbrayata, N. Lissaca, R. Ja®rezic and L. Ponsonnet: Mater. Sci. Eng. C, 2005, 25, 51.
[27] K. Smetana Jr: Biomaterials, 1993, 14, 1046.
[28] L. Chou, J. D. Firth, V. Uitto and D.M. Brunette: J. Cell Sci., 1995, 108, 1563.
[29] G. Caron-Lormier and H.Berry: J. Theor. Biol., 2005, 232, 235.
[30] X.F. Walboomers, H.J.E. Croes, L.A. Ginsel and J.A. Jansen: Biomaterials, 1998, 19, 1861.
[31] M. Eastwood, D.A. McGrowther and R.A. Brown: Proc. Inst. Mech. Eng., 1998, 212, 85.
[32] R.T. Prajapati, B. Chavally-Mis, D. Herbage, M. Eastwood and R.A. Brown: Wound Repair Regen., 2000, 8, 226.
[33] A.S.G. Curtis: Biomechanics and Cells, eds. F. Lyall and A.J. El Haj, Cambridge University Press, Cambridge, 1994, 121-130.
[34] G. Goldspink, A. Scutt, P. T. Loughna, D.J. Wells, T. Jaenicke and G.F. Gerlach: Am. J. Physiol., 1992, 262, R356.
[35] A.S.G. Curtis and G.M. Seehar: Nature, 1978, 274, 52.
[36] A.J. Maniotis, C.S. Chen and D.E. Ingber: Proc. Natl. Acad. Sci., 1997, 94, 849.
[37] B. Wojciak-Stothard, A. Curtis, W. Monaghan, K. MacDonald and C. Wilkinson: Exp. Cell Res., 1996, 223, 426.
[38] R. Carbone, L. Marangi, A. Zanardi, L. Giorgetti, E. Chierici, G. Berlanda, A. Podesta, F. Fiorentini, G. Bongiorno, P. Piseri, P.G. Pelicci and P. Milani: Biomaterials, 2006, 27, 3221.

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