3D printed porous titanium filled with mineralized UV-responsive chitosan hydrogel promotes cell proliferation and osteogenesis in vitro

doi:10.1016/j.jmst.2022.10.008

Abstract

Abstract: The modification of 3D printed porous titanium (Ti), especially for the internal pore structure, is critical and has received more attention to promoting its osteogenesis for clinical use. Ultra-violet (UV) responsive chitosan (CSMA), as an injectable filling material, was firstly incorporated into porous Ti, and then CSMA was in-situ mineralized by carbon oxide (CO₂) diffusion (CSMA/CaCO₃). Their physical-chemical and biological properties were investigated in vitro. CaCO₃ crystals within CSMA hydrogels were successfully deposited into pores of porous Ti, which exhibited favorable biocompatibility. Ti implants filled with CSMA/CaCO₃ promoted adhesion and proliferation of bone mesenchymal stem cells (BMSCs). Moreover, Ti implant filled CSMA/CaCO₃ hydrogels could increase alkaline phosphatase (ALP) activities, up-regulate osteopontin (OPN) and osteocalcin (OCN) expression levels, and enhance extracellular mineralization. 3D printed porous Ti filled with mineralized UV-responsive chitosan hydrogel could promote proliferation and osteogenesis of BMSCs, and have great potential for the modification of porous Ti implants in bone tissue engineering.

Key words: Porous titanium, Chitosan, Ultraviolet (UV)-responsive, Mineralization, Osteogenesis

Jiazhao Yang, Fan Liu, Changshun Zhou, Hejie Li, Gaolin Yang, Shiyuan Fang, In-Seop Lee, Yi Liu, Hao Bai, Cen Chen. 3D printed porous titanium filled with mineralized UV-responsive chitosan hydrogel promotes cell proliferation and osteogenesis in vitro[J]. J. Mater. Sci. Technol., 2023, 142: 34-44.

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