Enhanced Performance of Osteoblasts by Silicon Incorporated Porous TiO2 Coating

Abstract

Abstract: Silicon (Si) incorporated porous TiO₂ coating (Si-TiO₂) prepared on titanium (Ti) by micro-arc oxidation (MAO) technique was demonstrated to be cytocompatible in previous studies. In view of the potential clinical applications, a detailed in vitro study of the biological activity of Si-TiO₂ coating, in terms of osteoblast (MC3T3-E1 cells) morphology, proliferation, differentiation and mineralization was performed. Immunofluorescent staining indicated that cells seeded on the Si-TiO₂ coating showed improved adhesion with developing mature cytoskeletons, which contained numerous distinct and well-defined actin stress fibers in the cell membranes compared with those on the TiO₂ coating and Ti plate. Results from proliferation assay showed that the proliferation rate of cells seeded on the Si-TiO₂coating was significantly faster than that on the TiO₂ coating and Ti plate. Furthermore, the analysis of osteogenic gene expression demonstrated that the Si-TiO₂ coating stimulated the expression of osteoblast-related genes and promoted differentiation and mineralization of MC3T3-E1 cells. In addition, the Si-TiO2 coating differentially regulated Wnt signaling pathway by up-regulating the expression of low-density lipoprotein (LDL) receptor-related protein 5 (Lrp5), and downregulating the expression of Dickkopf-1 (Dkk1). All together, these results indicate that the investigated
titanium with Si-TiO₂ coating is biocompatible and a good candidate material used as implants.

Key words: Micro-arc oxidation, Silicon, TiO₂, Osteoblast, Gene expression

Quanming Wang, Hongjie Hu, Yuqing Qiao, Zhengxiang Zhang, Junying Sun. Enhanced Performance of Osteoblasts by Silicon Incorporated Porous TiO₂ Coating[J]. J Mater Sci Technol, 2012, 28(2): 109-117.

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Enhanced Performance of Osteoblasts by Silicon Incorporated Porous TiO₂ Coating

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