Ovalbumin-loaded paramagnetic nano-triangles for enhanced dendritic cell stimulation, T1-MR imaging, and antitumor immunity

doi:10.1016/j.jmst.2022.11.024

J. Mater. Sci. Technol. ›› 2023, Vol. 148: 123-137.DOI: 10.1016/j.jmst.2022.11.024

• Research article • Previous Articles Next Articles

Ovalbumin-loaded paramagnetic nano-triangles for enhanced dendritic cell stimulation, T₁-MR imaging, and antitumor immunity

Yike Hou^a, Zhe Tang^a, Jabeen Farheen^a, Madiha Saeed^b, Lijia Luo^c, Wenzi Ren^c, Dandan Luo^a, Asim Mushtaq^a, Ruibo Zhao^a,d,*, Jian Ge^e, Zhangsen Yu^f, Yao Li^a, M. Zubair Iqbal^a,*, Xiangdong Kong^a,*

^aInstitute of Smart Biomedical Materials, School of Materials Science and Engineering, Zhejiang Sci-Tech University, Hangzhou 310018, China;
^bState Key Laboratory of Drug Research & Center of Pharmaceutics, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China;
^cCixi Institute of Biomedical Engineering, CAS Key Laboratory of Magnetic Materials and Devices, Key Laboratory of Additive Manufacturing Materials of Zhejiang Province, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China;
^dDepartment of Materials, Imperial College London, London SW7 2AZ, UK;
^eCollege of Life Sciences, China Jiliang University, Hangzhou 310018, China;
^fLaboratory of Nanomedicine, Medical Science Research Center, School of Medicine, Shaoxing University, Shaoxing 312000, China

Received:2022-05-03 Revised:2022-05-03 Accepted:2022-05-03 Published:2023-06-10 Online:2023-06-05
Contact: *E-mail addresses:. rzhao@zstu.edu.cn (R. Zhao), zubair@zstu.edu.cn (M.Z. Iqbal), kongxd@zstu.edu.cn (X. Kong)

Abstract

Abstract: Vaccine-based cancer immunotherapy has demonstrated a significant potential for cancer treatment in clinics. Although the efficiencies of vaccines are limited, they can be enhanced by a well-designed antigen delivery system that promotes sufficient antigen presentation of dendritic cells (DCs) for initiating high T cell immunity. Herein, antigen-loaded manganese oxide (Mn₃O₄) triangular-shaped ultrasmall nanoparticles were prepared to stimulate DC-based immunotherapy under the guidance of T₁ magnetic resonance imaging. The FDA-approved triblock copolymer Pluronic® F-68 was used not only to transfer the phase from hydrophobic to hydrophilic but also to enrich antigen loading and improve the biocompatibility of the prepared nanoparticles. Ovalbumin (OVA), a model antigen, was adsorbed on the surface of polymer-coated nanoparticles through electrostatic interaction to form Mn₃O₄@PF68-OVA nanoparticle-antigen complexes to stimulate DC-based immunization and antigen-specific T cell immunity. The Mn₃O₄@PF68-OVA nanovaccine (NV) induces negligible toxicity effects against 4T1 and bone marrow-derived dendritic cells (BMDCs) by conventional methods supports the proliferation of intestine organoids, which are an innovative three-dimensional cytotoxicity evaluation system, thereby indicating their potential safety for in vivo cancer therapies. The designed paramagnetic nanovaccine possessed excellent OVA delivery to dendritic-regulated antigen-specific T cells in vitro by stimulating the maturation level of BMDCs. In addition, Mn₃O₄@PF68-OVA NVs enhance immunity in vivo by increasing the T-cells and M1 macrophages, which suggests improved immunity. Excitingly, vaccination with Mn₃O₄@PF68-OVA offer complete protection in the prophylactic group and significant tumor inhibition in the therapeutic group against B16-OVA tumor. In addition, the designed nanovaccine demonstrated high T₁-MR imaging in the tumor, further justifying enhanced tumor accumulation and capability to real-time monitor the treatment procedure. This study presents a promising nanosystem to design an effective nanovaccine for T₁-MR imaging-guided tumor immunotherapy.

Key words: Ovalbumin, Nanovaccine, Antigen delivery, Magnetic resonance imaging, Contrast agent, Cancer immunotherapy

Yike Hou, Zhe Tang, Jabeen Farheen, Madiha Saeed, Lijia Luo, Wenzi Ren, Dandan Luo, Asim Mushtaq, Ruibo Zhao, Jian Ge, Zhangsen Yu, Yao Li, M. Zubair Iqbal, Xiangdong Kong. Ovalbumin-loaded paramagnetic nano-triangles for enhanced dendritic cell stimulation, T₁-MR imaging, and antitumor immunity[J]. J. Mater. Sci. Technol., 2023, 148: 123-137.

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Ovalbumin-loaded paramagnetic nano-triangles for enhanced dendritic cell stimulation, T₁-MR imaging, and antitumor immunity

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