Fabrication of multifunctional polydopamine-coated gold nanobones for PA/CT imaging and enhanced synergistic chemo-photothermal therapy

doi:10.1016/j.jmst.2020.04.060

Abstract

Abstract:

Multimodal imaging-guided chemo-photothermal therapy is an excellent cancer treatment, which can not only efficiently against tumor, but also can offer precise treatment window and real-time monitoring of the treatment efficiency. In our work, polydopamine (PDA)-coated gold nanobones (AuNBs@PDA nanocomplexes) were designed for this approach. The AuNBs@PDA nanocomplexes have strong absorbance in the near infrared (NIR) region and higher photothermal conversion efficiency (75.48 %) than gold nanobones alone, which was facilitated for photoacoustic imaging and photothermal therapy. Besides, the loading efficiency of doxorubicin (DOX) by AuNBs@PDA nanocomplexes could be up to about 70 % and DOX release from AuNBs@PDA/DOX nanocomplexes sensitively response to the lower pH environment and NIR laser irradiation, which makes them become the excellent nano-carrier for the delivery of chemotherapy drug. In vitro and in vivo studies showed significant cytotoxicity and antitumor efficacy by the AuNBs@PDA/DOX nanoplatform with negligible side effects. Meanwhile, the nanoplatform was also successfully employed for computed tomography (CT) imaging, attributing to the high atomic number and high X-ray attenuation coefficient of gold. Therefore, we believed that the proposed PDA-coated gold nanobones would be a novel multifunctional theranostic nanoagent to realize the PA/CT imaging-guided chemo-photothermal therapy of cancer.

Key words: Imaging, Photothermal therapy, Multifunctional nanobones

Jingwei Xu, Xiaju Cheng, Fuxian Chen, Weijie Li, Xiaohui Xiao, Puxiang Lai, Guopeng Xu, Li Xu, Yue Pan. Fabrication of multifunctional polydopamine-coated gold nanobones for PA/CT imaging and enhanced synergistic chemo-photothermal therapy[J]. J. Mater. Sci. Technol., 2021, 63: 97-105.

Figures/Tables 5

Scheme 1. (a) The diagram of the AuNBs@PDA/DOX nanocomplexes preparation; (b) The AuNBs@PDA/DOX nanocomplexes used for PA/CT imaging-guided chemo-photothermal therapy of cancer.

Fig. 1. (a, b) TEM images of AuNBs and AuNBs@PDA; (c) Vis-NIR absorption spectra of AuNBs and AuNBs@PDA; (d) The photothermal heating curves of AuNBs@PDA nanocomplexes with different concentrations under 808 nm laser irradiation (1 W/cm2) for 5 min; (e) Quantification of DOX loading at different feeding amounts of DOX; (f) DOX release from AuNBs@PDA/DOX nanocomplexes overtime in different pH values (7.2 and 6) buffers and DOX release from AuNBs@PDA/DOX nanocomplexes at pH values of 6 with NIR laser irradiation.

Fig. 2. Chemo-photothermal therapy for cancer in vitro. (a)-(c) Cell viability of 4T1 cells after different treatments (NIR = 808 nm laser, 1 W/cm2, 5 min, CDOX =6.6 μg/mL, CAuNBs@PDA =50 μg/mL); (d) Live/dead fluorescence staining of 4T1 cells after different treatments. Scale bar =50 μm.

Fig. 3. (a) PA images with different concentrations AuNBs@PDA nanocomplexes; (b) in vivo PA images of tumor bearing mice after intravenous injection with AuNBs@PDA nanocomplexes (2 mg/mL, 100 μL) acquired at different time points (inset: PA signal of tumor site); (c) CT images of the left and right tumor after intratumoral injection with AuNBs@PDA nanocomplexes and iopromide, respectively.

Fig. 4. (a, b) Photothermal images and temperature changes of tumor-bearing mice under 808 nm laser irradiation (1 W/cm2) after injection with PBS or AuNBs@PDA nanocomplexes (2 mg/mL, 100 μL); (c, d) Tumor volume growth curves and tumor growth inhibition ratio of mice after receiving different therapeutic treatments; (e) Slices of tumor tissues obtained and stained with H&E after receiving different therapeutic treatments, scale bar = 100 μm.

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