Mediating Mesenchymal Stem Cells Responses and Osteopontin Adsorption via Oligo(ethylene glycol)-amino Mixed Self-assembled Monolayers

doi:10.1016/j.jmst.2016.04.005

J. Mater. Sci. Technol. ›› 2016, Vol. 32 ›› Issue (9): 966-970.DOI: 10.1016/j.jmst.2016.04.005

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Mediating Mesenchymal Stem Cells Responses and Osteopontin Adsorption via Oligo(ethylene glycol)-amino Mixed Self-assembled Monolayers

Hao Lijing^1,²,Li Tianjie^1,²,Zhao Naru^1,²,Cui Fuzhai³,Du Chang^1,^2,^*(),Wang Yingjun^1,^2,^*()

¹ School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China
² National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China
³ Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, China

Received:2015-09-22 Accepted:2015-11-06 Online:2016-09-20 Published:2016-11-02
Contact: Du Chang,Wang Yingjun

Abstract

Abstract:

Oligo(ethylene glycol) (-OEG) and amino (-NH₂) mixed self-assembled monolayers (SAMs) were employed as model substrates to investigate the effect of charge density on the fate of mesenchymal stem cells (MSCs) and osteopontin (OPN) adsorption. We found that all surfaces presenting -NH₂ groups favored cell responses regardless of the surface charge. Meanwhile, OPN adsorption could remain stable on the mixed SAMs over a certain range of charge densities. Our work provides some insights into cell responses and protein adsorption to surface charge.

Key words: Mixed self-assembled monolayers, Oligo(ethylene glycol)-amino, Mesenchymal stem cell, Osteopontin

Hao Lijing,Li Tianjie,Zhao Naru,Cui Fuzhai,Du Chang,Wang Yingjun. Mediating Mesenchymal Stem Cells Responses and Osteopontin Adsorption via Oligo(ethylene glycol)-amino Mixed Self-assembled Monolayers[J]. J. Mater. Sci. Technol., 2016, 32(9): 966-970.

Figures/Tables 4

Fig. 1. Surface characterization of the -OEG/-NH2 mixed SAMs: (a) S2p XPS spectra; (b) FTIR-ATR; (c) typical XPS spectra; (d) water contact angle, and (e) Zeta potential.

Table 1 Percentage of surface -NH2, IEP, water contact angles, OPN mass and retention of -OEG/-NH2 mixed SAMs

-OEG/-NH₂ mixed SAMs	v/v solution
-OEG/-NH₂ mixed SAMs	-OEG	7/3	5/5	3/7	-NH₂
Surface -NH2(%)	0	41.93	60.15	71.94	1
IEP	4.02	4.49	5.28	4.80	6.20
Contact angle (deg.)	38.20?±?1.21	48.25?±?4.12	57.60?±?1.7	69.58?±?5.01	68.38?±?2.01
OPN mass (ng cm-2)	3.07?±?0.63	47.09?±?3.99	40.97?±?4.35	50.67?±?5.72	90.69?±?13.62
OPN retention (%)	71.04?±?16.19	60.09?±?4.00	60.17?±?2.58	59.80?±?3.59	73.42?±?4.66

Fig. 2. (a) The morphology, (b) proliferation and (c) relative gene expression of Runx-2, ALP, osteocalcin and collagen Ι of mMSCs on the -OEG/-NH2 mixed SAMs. Cells were stained for F-actin and nuclei with AlexaFluor 488 phalloidin (green) and DAPI (blue), respectively. ★indicates to be statistically significant (p?<?0.05).

Fig. 3. (a) OPN adsorption and retention onto the -OEG/-NH2 mixed SAMs; (b) effect of -OEG/-NH2 mixed SAMs on OPN mAb was quantified by ELISA; and (c) morphology of mMSCs on pre-adsorbed OPN onto the -OEG/-NH2 mixed SAMs after 12?h of culture in serum-free medium. ★indicates to be statistically significant (p?<?0.05).

Mediating Mesenchymal Stem Cells Responses and Osteopontin Adsorption via Oligo(ethylene glycol)-amino Mixed Self-assembled Monolayers

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