Biomimetic melatonin-loaded silk fibroin/GelMA scaffold strengthens cartilage repair through retrieval of mitochondrial functions

doi:10.1016/j.jmst.2022.10.031

Abstract

Abstract: Articular cartilage injury induced by collision or trauma is a common sports-related condition that may progress to pain, dysfunction, and secondary osteoarthritis (OA). Limited by self-renewal potential, cartilage regeneration faces a quandary while bio-inspired novel strategies are urgently required. In this study, by a soft freezing method and surface modification technique, a multi-functional silk fibroin (SF) plus gelatin methacrylate (GelMA) scaffold laden with melatonin (MT) was prepared. SF-GelMA@MT scaffold demonstrated enhanced biomechanical characteristics and long-acting melatonin release. In vitro treatment with SF-GelMA@MT induced the synthesis of cartilage extracellular matrix (ECM) components. Mechanistically, sustained release of melatonin yielded robust chondroprotective effects via improving mitochondrial polarization and antioxidant properties. SF-GelMA@MT implantation boosted cartilage renascence in a full-thickness cartilage defect model via mitochondria-associated sirtuins 1 (SIRT1)-superoxide dismutase 2 (SOD2) signaling pathway in vivo. In summary, this research proposed a well-designed bionic composite scaffold that promotes cartilage regeneration via mitochondrial function enhancement, which is of tremendous potential for cartilage tissue engineering.

Key words: Cartilage injury, Silk fibroin, Gelatin methacrylate, Melatonin, Mitochondria

Mingzhuang Hou, Yijian Zhang, Yang Liu, Xiaoyang Ge, Xiayu Hu, Zhijian Zhao, Xin Tian, Tao Liu, Huilin Yang, Xi Chen, Fan He, Xuesong Zhu. Biomimetic melatonin-loaded silk fibroin/GelMA scaffold strengthens cartilage repair through retrieval of mitochondrial functions[J]. J. Mater. Sci. Technol., 2023, 146: 102-112.

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