Abstract:

Diabetes, one of the most serious metabolism diseases, has continually plagued human beings so that timely monitored blood glucose level is very necessary. Gold nanoparticles (AuNPs) have been considered as potential substitutes for non-enzymatic sensors, due to their unusual physicochemical property and efficient catalytic activity. Herein, we prepared a facial platform for AuNPs to maintain good dispersity and inherent physicochemical property. In this special platform (SH-nGO-PEG-RhB), nanographene oxide (nGO) was employed as an ideal base, which provided adequate carboxyls for conjugating with poly(ethylene glycol) (PEG), and enough epoxy groups for thiol active site. A functional dye (rhodamine B) with preferable fluorescence properties was also introduced into this platform. AuNPs were then aptly in situ grown on the platform via reduction of HAuCl₄ under mild conditions, providing AuNPs@SH-nGO-PEG-RhB. The nanocomposite was cast on a glassy carbon electrode (GCE) conveniently, yielding GCE/AuNPs@SH-nGO-PEG- RhB. Reasonably, as shown by cyclic voltammetry (CV) and ready-state amperometry, GCE/AuNPs@SH-nGO-PEG-RhB was capable of electrochemical determination of glucose so as to provide an efficient sensor for glucose determination with dye tracer.

Key words: Nano-graphene oxide, Gold nanoparticles, Glucose, Biosensor, Rhodamine B