J. Mater. Sci. Technol. ›› 2021, Vol. 91: 90-104.DOI: 10.1016/j.jmst.2021.02.047

• Review Article • Previous Articles     Next Articles

In vitro and in vivo evaluation of implantable bacterial-killing coatings based on host defense peptides and their synthetic mimics

Yuxin Qiana, Shuai Dengb, Xue Wub, Yunrui Sheb, Runhui Liua,b, Haodong Linc,*()   

  1. aState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
    bKey Laboratory for Ultrafine Materials of Ministry of Education, Frontiers Science Center for Materiobiology and Dynamic Chemistry, Research Center for Biomedical Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China
    cDepartment of Orthopedic Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China
  • Received:2020-12-09 Revised:2021-01-17 Accepted:2021-02-02 Published:2021-11-20 Online:2021-11-20
  • Contact: Haodong Lin
  • About author:*E-mail address: haodonglin@hotmail.com (H. Lin).
    First author contact:§These authors contributed equally to this review.

Abstract:

Contact-killing antimicrobial coatings based on host defense peptides (HDPs) and their synthetic mimics have shown potential as powerful tools to combat implant-associated infections. Covalent modification of the antimicrobial surface has been utilized to prevent early-stage microbial infections owing to the less drug-leaching possibility that is beneficial to human health and the natural environment. Although considerable progress has been achieved in preparing contact-killing antimicrobial surfaces, discussions focusing on the in vitro and in vivo evaluations of these surfaces are limited. In this review, we summarized the established in vitro methods to simulate the practical interaction of microbes with the surrounding biological environment and the reported in vivo studies at different implant sites. We suggested that the in vivo specific site infection model is essential to gain a comprehensive understanding of these antimicrobial coatings in the preclinical stage, which can be established based on investigations performed using various in vitro assays and conventional non-specific site infection models. Overall, these precedent studies focusing on bacterial contact-killing coatings modified with HDPs and HDP mimics can be considered as critical to assess the surface antibacterial ability and to guide the future developments and applications of antimicrobial surfaces.

Key words: Contact-killing, Antimicrobial surface, HDPs, HDP mimic, Covalent binding