Fe3O4@silica nanoparticles for reliable identification and magnetic separation of Listeria monocytogenes based on molecular-scale physiochemical interactions

doi:10.1016/j.jmst.2021.01.014

J. Mater. Sci. Technol. ›› 2021, Vol. 84: 116-123.DOI: 10.1016/j.jmst.2021.01.014

• Research Article • Previous Articles Next Articles

Fe₃O₄@silica nanoparticles for reliable identification and magnetic separation of Listeria monocytogenes based on molecular-scale physiochemical interactions

Huawei Rong^a^,^b, Tong Gao^a, Yun Zheng^a, Lingwei Li^b, Dake Xu^a, Xuefeng Zhang^a^,^c^,^**(), Yanglong Hou^d^,^*(), Mi Yan^c^,^e

^aKey Laboratory for Anisotropy and Texture of Materials (MOE), School of Materials Science and Engineering, Northeastern University, Shenyang 110819, China
^bKey Laboratory of Electromagnetic Processing of Materials (Ministry of Education), School of Materials Science and Engineering, Northeastern University, Shenyang 110819, China
^cInstitute of Advanced Magnetic Materials, College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou 310012, China
^dBeijing Key Laboratory for Magnetoelectric Materials and Device (BKLMMD), Beijing Innovation Center for Engineering Science and Advanced Technology (BIC-ESAT), Department of Materials Science and Engineering, College of Engineering, Peking University, Beijing 100871, China
^eState Key Laboratory of Silicon Materials, School of Materials Science and Engineering, Zhejiang University, Hangzhou 310027, China

Received:2020-10-11 Revised:2020-10-29 Accepted:2020-11-03 Published:2021-09-10 Online:2021-01-09
Contact: Xuefeng Zhang,Yanglong Hou
About author:* hou@pku.edu.cn (Y. Hou).
** Key Laboratory for Anisotropy and Texture of Mate-rials (MOE), School of Materials Science and Engineering, Northeastern University, Shenyang 110819, China. E-mail addresses: zhangxf@atm.neu.edu.cn (X. Zhang),

Abstract

Abstract:

Listeria monocytogenes (L. monocytogenes) is one of the top five dangerous foodborne pathogens which widely exists in most raw food and has approximately 30 % mortality rate in high-risk groups. Food safety caused by foodborne pathogens is still a major problem faced by humans in all world. The conventional analytical methods currently used involve complex bacteriological tests and usually take several days for incubation and analysis. Thus, in order to prevent the spread of disease, the development of a detection method with high speed, high accuracy and sensitivity is urgent and necessary. Herein, we developed an approach for the identification and magnetic capture of L. monocytogenes by using core@shell Fe₃O₄@silica nanoparticles terminated with hydroxyl or amine groups. Our results show that both amine- and hydroxyl-terminated Fe₃O₄@silica core@shell nanoparticles functionalized with specific antibodies, present 95.2 %±6.2 % and 98.6 %±0.3 % capture efficacies, respectively. However, without conjugating the specific antibodies, the hydroxyl-terminated Fe₃O₄@silica nanoparticles exhibit 17.6 %±1.6 % efficacy, while the amine-terminated one remains 93.2 %±9.2 % capture efficiency ascribed to the high affinity. This study quantitatively uncovers the specific and non-specific recognitions relevant to the molecular-scale physiochemical interactions between the microorganisms and the functionalized particles, and the results from this work can be generalized and extended to other bacterial species by changing antibodies, also have important implications in developing advanced analytic methods.

Key words: Listeria monocytogenes, Magnetic capture, Fe₃O₄@silica, Functionalized nanoparticles

Huawei Rong, Tong Gao, Yun Zheng, Lingwei Li, Dake Xu, Xuefeng Zhang, Yanglong Hou, Mi Yan. Fe₃O₄@silica nanoparticles for reliable identification and magnetic separation of Listeria monocytogenes based on molecular-scale physiochemical interactions[J]. J. Mater. Sci. Technol., 2021, 84: 116-123.

Figures/Tables 6

Fig. 1. Sketch of amine- and hydroxyl-terminated Fe3O4@silica nanoparticles without and with specific antibodies for the capture of L. monocytogenes under the incubation time of 30 min at 37 ℃. The results show the selective conjugation between the L. monocytogenes and the functionalized nanoparticles.

Fig. 2. TEM images of (a) Fe3O4, (b) Fe3O4@silica nanoparticles, (c) L. monocytogenes, (d) L. monocytogenes conjugated with antibody-functionalized Fe3O4@silica nanoparticles (yellow dots) and (e) the capture of L. monocytogenes (green ribbon) by using a small SmCo magnet.

Fig. 3. SEM (a, d, g, and j), TEM (b, e, h, and k) and high-magnified TEM (c, f, i, and l) images of L. monocytogenes conjugated with Fe3O4@silica nanoparticles: (a-c) Fe3O4@silica-OH, (d-f) Anti-Fe3O4@silica-OH, (g-i) Fe3O4@silica-NH2 and (j-l) Anti-Fe3O4@silica-NH2.

Fig. 4. Efficiencies of magnetic isolation of L. monocytogenes by hydroxyl- and amine-terminated Fe3O4@silica nanoparticles functionalized with and without specific antibodies at the incubation temperature of 37 ℃: (a, b) the incubation time is 10 min with mild mixing, (c, d) the incubation time is 30 min with mild mixing.

Fig. 5. Efficiencies of magnetic isolation of L. monocytogenes by hydroxyl- and amine-terminated Fe3O4@silica nanoparticles functionalized with and without specific antibodies at the incubation temperature of 25 ℃: (a, b) the incubation time is 10 min with mild mixing, (c, d) the incubation time is 30 min with mild mixing.

Fig. 6. (a, b) Comparison of the isolation efficiencies of four kinds of nanoparticles at a nanoparticle concentration of 200 μg/mL with the incubation time at the incubation temperature of 25 ℃ and 37 ℃, respectively; (c, d) the enhancement fractions of anti-Fe3O4@silica-OH and anti-Fe3O4@silica-NH2 with the incubation time at the incubation temperature of 25 ℃ and 37 ℃, respectively.

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Fe₃O₄@silica nanoparticles for reliable identification and magnetic separation of Listeria monocytogenes based on molecular-scale physiochemical interactions

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