J. Mater. Sci. Technol. ›› 2012, Vol. 28 ›› Issue (7): 647-653.

• Reviews • 上一篇    下一篇

Cytocompatibility Study of High Nitrogen Nickel-free Stainless Steel

马天驰1,万鹏2,崔煜艳3,张桂荣4,李济强4,刘继辉4,任伊宾2,杨柯2,卢利1   

  1. 1. 中国医科大学口腔医学院
    2. 中国科学院金属研究所
    3. 沈阳第四人民医院
    4. 沈阳市口腔医院
  • 收稿日期:2011-07-04 修回日期:2011-09-22 出版日期:2012-07-28 发布日期:2012-07-28
  • 通讯作者: 卢利
  • 基金资助:

    国家自然科学基金;中科院知识创新工程

Cytocompatibility of High Nitrogen Nickel-Free Stainless Steel for Orthopedic Implants

Tianchi Ma1,4), Peng Wan2), Yuyan Cui3), Guirong Zhang4), Jiqiang Li4), Jihui Liu4), Yibin Ren2), Ke Yang2),  Li Lu1)   

  1. 1) School of Stomatology, China Medical University, Shenyang 110002, China
    2) Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, China
    3) Shenyang the Fourth Hospital of People, Shenyang 110032, China
    4) Shenyang Stomatoiogy Hospital, Shenyang 110002, China
  • Received:2011-07-04 Revised:2011-09-22 Online:2012-07-28 Published:2012-07-28
  • Contact: Li Lu
  • Supported by:

    the National Natural Science Foundation of China (No. 31000428) and the National Basic Research Program of China (\973 Program", No. 2012CB619101)

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摘要： cytocompatibility of high nitrogen nickel-free stainless steel (HNS) with different nitrogen content was evaluated and compared with a conventional austenitic stainless steel 317L. The MTT test was performed on MG63 osteoblasts to assess the cytotoxicity. The expression of selected marker typical of differentiated osteoblasts, such as alkaline phosphatase activity (AKP), was also monitored in MG63 cells cultured on the tested materials. As a result, HNS had higher cell growth than 317L; meanwhile the improvement was increased with the nitrogen content. Furthermore, HNS enhanced osteoblasts differentiation, as confirmed by AKP activity. Overall these facts indicate that HNS has higher cytocompatibility than 317L and that the nitrogen content contributes to the higher cytocompatibility of HNS. The influence of nitrogen on surface energy further explains the cytocompatibility of HNS.

Abstract: Cytocompatibility of high nitrogen nickel-free stainless steel (HNS) with different nitrogen content was evaluated and compared with a conventional austenitic stainless steel 317L. The MTT assay (3-(4,5-dimethylthiazol2-yl)-2,5-diphenyltetrazolium bromide) was performed on MG63 osteoblasts to assess the cytotoxicity. The expression of selected marker typical of differentiated osteoblasts, such as alkaline phosphatase activity (AKP), was also monitored in MG63 cells cultured on the tested materials. As a result, HNS had higher cell growth than 317L; meanwhile the cytocompatibility was increased with increasing nitrogen content. Furthermore, HNS enhanced osteoblasts differentiation, as confirmed by AKP activity. Overall these facts indicated that HNS had higher cytocompatibility than 317L and the nitrogen content contributed to the higher cytocompatibility of HNS. The influence of nitrogen on surface energy further explained the cytocompatibility of HNS.

Key words: High nitrogen stainless steel, Cytoxicity evaluation, Osteoblasts, Bone, Surface energy