Nanomaterial Labels in Lateral Flow Immunoassays for Point-of-Care-Testing

Jiuchuan Guo, Shuqin Chen, Jinhong Guo, Xing Ma

Table 3 Comparison between CG-LFIAs and alternate nanoparticles labelled LFIAs in terms of sensitivity and quantification.

Target Analyte	Detection Sensitivity and Quantitative Range
Target Analyte	Gold Standard (CG-LFIAs)	FLFIAs (QDs, UCPs, TRFNPs)	SERS-LFIAs	MLFIAs	CLFIAs
ENR	0.138 μg/kg, none	1 μg/L, none (QDs)
AIV	AIV(NDV) sample diluted to 2^-12 (2^-10), none	0.09 ng/mL, 0.27-12 ng/mL (QDs)
DON	100 μg/kg, none	50 μg/kg, none (QDs)			20 μg/kg, 18.75 - 600 μg/kg
ZEN	20 ng/mL, 5-200 ng/mL	59.15 pg/mL, 0.1-15 ng/mL (QDs)			1 μg/kg 0.938 - 30 μg/kg
AFB1	0.5 ng/mL, none	1.65 pg/mL, 2-300 pg/mL (QDs)
AFB1	0.5 ng/mL, none	0.03 ng/mL, 0.03-1000 ng/mL (UCPs)
V. cholerae O139	1 cfu/mL, none	10⁴ CFU/mL, 10⁵-10⁸ CFU/mL (UCPs)
PVX	0.3 ng/mL, 0.3-80 ng/mL			0.25 ng/mL, 0.25-16 ng /mL
AFP	0.3 ng/mL, none	0.05 ng/mL, 0.1-750 ng/mL (TRFNPs)
hCG	2.3 mIU/mL, 10-120 mIU/mL	10 pg/100 μl, none (UCPs)	1.6 mIU/mL, 1.6-1679 mIU/mL	45.6 mIU/mL, 45.6-230 mIU/mL
hCG	2.3 mIU/mL, 10-120 mIU/mL	0.08 ng/mL, 0.16-450 ng/mL (TRFNPs)	1.6 mIU/mL, 1.6-1679 mIU/mL	45.6 mIU/mL, 45.6-230 mIU/mL
CEA	4.6 pg/mL, 0.01-50 ng/mL	0.35 ng/mL, 2.8-680 ng/mL (QDs)		0.045 ng/mL, 0.045-100 ng/mL
NEO	100 ng/mL, 0-50 ng/mL		0.37 pg/mL, 1-1000 pg/mL
RAC	40.1 pg/mL, 25-2500 pg/mL	23.6 pg/mL, 25-2500 pg/mL (QDs)
RAC	40.1 pg/mL, 25-2500 pg/mL	7.2 pg/mL, 5-2500 pg/mL (TRFNPs)
SEB	1ng/mL, none		0.001 ng/mL, none
L. monocytogenes	3.9 × 10⁵ CFU/mL, none		19 CFU/mL, 19-1.9 × 10⁴ CFU/mL	3.5 × 10³ CFU/mL, none