J. Mater. Sci. Technol. ›› 2021, Vol. 69: 188-199.DOI: 10.1016/j.jmst.2020.08.021
• Research Article • Previous Articles Next Articles
Wei Fenga,b, Nian Liua,b, Lingling Gaoa,b, Qian Zhoub, Luofeng Yua,b, Xiaoting Yeb, Jingjing Huob, Xiao Huanga,b, Peng Lia,b,c,*(), Wei Huanga,b
Received:
2020-05-20
Revised:
2020-06-15
Accepted:
2020-06-23
Published:
2021-04-10
Online:
2021-05-15
Contact:
Peng Li
About author:
*Frontiers Science Center for Flexible Electronics (FSCFE), Xi’an Institute of Flexible Electronics (IFE) & Xi’an Institute of Biomedical Materials and Engineering (IBME), Northwestern Polytechnical University (NPU), Xi’an 710072, China E-mail address: iampli@nwpu.edu.cn (P. Li).Wei Feng, Nian Liu, Lingling Gao, Qian Zhou, Luofeng Yu, Xiaoting Ye, Jingjing Huo, Xiao Huang, Peng Li, Wei Huang. Rapid inactivation of multidrug-resistant bacteria and enhancement of osteoinduction via titania nanotubes grafted with polyguanidines[J]. J. Mater. Sci. Technol., 2021, 69: 188-199.
Fig. 2. (a) FESEM images (scale bar = 200 nm), (b) water contact angles, (c) XPS spectra of Ti, Ti-PG, TNT and TNT-PG surfaces and (d) high-resolution N 1s XPS spectrum of TNT-PG.
Fig. 3. In vitro bactericidal activity tests. (a) Photographs of MRSA, P. aeruginosa and E. coli colonies formed on LB agar plates after incubation on the surfaces of TCPS, Ti, Ti-PG, TNT and TNT-PG. Killing ratio and log reduction of (b) MRSA, (c) P. aeruginosa and (d) E. coli on Ti, Ti-PG, TNT, TNT-PG surfaces. (e) Inhibition zones of Ti, Ti-PG, TNT and TNT-PG for MRSA.
Fig. 4. In vitro cytotoxicity assays of Ti, Ti-PG, TNT and TNT-PG with pre-osteoblasts (MC3T3-E1). (a) Fluorescent intensity (alamarBlue assay) of pre-osteoblasts on Ti, Ti-PG, TNT and TNT-PG surfaces relative to TCPS group at day 1, 4, and 7 (mean ± standard deviation). (b) LIVE/DEAD fluorescent images of pre-osteoblasts on the TCPS, Ti, Ti-PG, TNT and TNT-PG surfaces at day 1, 4, and 7 (scale bar =1 μm).
Fig. 5. Bacterial killing kinetics of (a) Ti-PG and (b) TNT-PG against MRSA, P. aeruginosa and E. coli. (c) FESEM images of bacterial morphology after incubation on the Ti, Ti-PG, TNT and TNT-PG surfaces.
Fig. 6. Killing ratio of TNT-PG toward 107 CFU mL-1 MRSA and E. coli after (a) 1 h ultrasonication and (b) 3-days PBS immersion. (c) Reusable antibacterial activity of TNT-PG coating by repeated challenges with 107 CFU mL-1 of E. coli up to ten cycles.
Fig. 7. In vivo anti-infective activity. (a) Photographs of rat implanted with Ti, Ti-PG, TNT and TNT-PG at day 0. (b) At day 5, the incisions were opened and the inflammation at the wound sites in four groups was visually observed. Viable MRSA retrieved from (c) the implant surfaces and (d) the skin tissues around the implants after 5 days implantation (*p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001).
Fig. 8. Histomorphological evaluation of the tissues around Ti, Ti-PG, TNT and TNT-PG implants (scale bar =100 μm, the black arrows indicate the inflammatory neutrophils).
Fig. 9. ALP expression and ECM mineralization of pre-osteoblasts on Ti, Ti-PG, TNT and TNT-PG surfaces under osteoinductive conditions: (a) representative images of ALP staining at day 4 and 7, (b) representative images of ARS staining (the circled parts indicate the calcium deposition) at day 7 and 14, (c) quantitation of calcium deposition at day 7 and 14 (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).
[1] |
D. Wang, G. He, Y. Tian, N. Ren, J. Ni, W. Liu, X. Zhang, J. Mater. Sci. Technol. 44 (2020) 160-170.
DOI URL |
[2] |
C.R. Arciola, D. Campoccia, L. Montanaro, Nat. Rev. Microbiol. 16 (2018) 397-409.
DOI URL |
[3] | A.F. Engelsman, I.C. Saldarriaga-Fernandez, M.R. Nejadnik, G.M. van Dam, K.P. Francis, R.J. Ploeg, H.J. Busscher, H.C. van der Mei, Biofouling 26 (2010) 761-767. |
[4] |
R.O. Darouiche, N. Engl. J. Med. 350 (2004) 1422-1429.
DOI URL |
[5] |
M.R. Streit, D. Haeussler, T. Bruckner, T. Proctor, M.M. Innmann, C. Merle, T. Gotterbarm, S. Weiss, Clin. Orthop. Relat. Res. 474 (2016) 1697-1706.
DOI URL |
[6] |
L. Tan, J. Li, X. Liu, Z. Cui, X. Yang, S. Zhu, Z. Li, X. Yuan, Y. Zheng, K. W.K.Yeung, H. Pan, X. Wang, S. Wu, Adv. Mater. 30 (2018), 1801808.
DOI URL |
[7] | S. Renvert, M. Quirynen, Clin. Oral Implants Res. 26 (2015) 15-44. |
[8] |
D. Li, Y. Li, A. Shrestha, S. Wang, Q. Wu, L. Li, C. Guan, C. Wang, T. Fu, W. Liu, Y. Huang, P. Ji, T. Chen, Adv. Healthcare Mater. 8 (2019), 1900002.
DOI URL |
[9] |
B. Li, J. Ma, D. Wang, X. Liu, H. Li, L. Zhou, C. Liang, H. Wang, Biomater. Sci. 7 (2019) 4075-4087.
DOI URL |
[10] |
Z. Jia, P. Xiu, M. Li, X. Xu, Y. Shi, Y. Cheng, S. Wei, Y. Zheng, T. Xi, H. Cai, Z. Liu, Biomaterials 75 (2016) 203-222.
DOI URL |
[11] |
H. Wang, J. Ji, W. Zhang, W. Wang, Y. Zhang, Z. Wu, Y. Zhang, P.K. Chu, Acta Biomater. 6 (2010) 154-159.
DOI URL |
[12] | R. Beranek, H. Tsuchiya, T. Sugishima, J.M. Macak, L. Taveira, S. Fujimoto, H. Kisch, P. Schmukic, Appl. Phys. Lett. 87 (2005), 243117. |
[13] |
B. Munirathinam, L. Neelakantan, Mater. Sci. Eng. C 49 (2015) 567-578.
DOI URL |
[14] |
N. Wang, H. Li, W. Lu, J. Li, J. Wang, Z. Zhang, Y. Liu, Biomaterials 32 (2011) 6900-6911.
DOI PMID |
[15] |
E.M. Lotz, R. Olivares-Navarrete, S. Berner, B.D. Boyan, Z. Schwartz, J. Biomed. Mater. Res. A 104 (2016) 3137-3148.
DOI URL |
[16] |
Z. Jin, X. Yan, K. Shen, X. Fang, C. Zhang, Q. Ming, M. Lai, K. Cai, Colloids Surf. B 181 (2019) 416-425.
DOI URL |
[17] |
B.D. Boyan, A. Cheng, R. Olivares-Navarrete, Z. Schwartz, Adv. Dent. Res. 28 (2016) 10-17.
DOI URL |
[18] |
J. Chen, X. Shi, Y. Zhu, Y. Chen, M. Gao, H. Gao, L. Liu, L. Wang, C. Mao, Y. Wang, Theranostics 10 (2020) 109-122.
DOI URL |
[19] |
S. Wu, Z. Weng, X. Liu, K. W.K.Yeung, P.K. Chu, Adv. Funct. Mater. 24 (2014) 5464-5481.
DOI URL |
[20] |
X. Li, M. Qi, X. Sun, M.D. Weir, F.R. Tay, T.W. Oates, B. Dong, Y. Zhou, L. Wang, H.H.K. Xu, Acta Biomater. 94 (2019) 627-643.
DOI URL |
[21] |
X. Jin, Y. Xiong, X. Zhang, R. Wang, Y. Xing, S. Duan, D. Chen, W. Tian, F. Xu, Adv. Funct. Mater. 29 (2019), 1807915.
DOI URL |
[22] |
D.I. Andersson, D. Hughes, Nat. Rev. Microbiol. 8 (2010) 260-271.
DOI URL |
[23] | K. Salimiyan Rizi, K. Ghazvini, M.K. Noghondar, J. Infect. Dis. Ther. 6 (2018), 1000363. |
[24] |
D. Campoccia, L. Montanaro, P. Speziale, C.R. Arciola, Biomaterials 31 (2010) 6363-6377.
DOI PMID |
[25] |
M. Ma, X. Liu, L. Tan, Z. Cui, X. Yang, Y. Liang, Z. Li, Y. Zheng, K. W.K.Yeung, S. Wu, Biomater. Sci. 7 (2019) 1437-1447.
DOI URL |
[26] |
Z. Zhi, Y. Su, Y. Xi, L. Tian, M. Xu, Q. Wang, S. Padidan, P. Li, W. Huang, ACS Appl. Mater. Interfaces 9 (2017) 10383-10397.
DOI URL |
[27] |
L.H. Yang, V.D. Gordon, D.R. Trinklea, N.W. Schmidtb, M.A. Davisa, C. DeVriesa, A. Som, J.E. Cronan, G.N. Tew, G.C.L. Wong, Proc. Natl. Acad. Sci. U. S.A. 105 (2008) 20595-20600.
DOI URL |
[28] | N.W. Schmidt, G.C. Wong, Curr. Opin. Solid State Mater.Sci. 17 (2013) 151-163. |
[29] |
M.K. Oule, R. Azinwi, A.M. Bernier, T. Kablan, A.M. Maupertuis, S. Mauler, R.K. Nevry, K. Dembele, L. Forbes, L. Diop, J. Med. Microbiol. 57 (2008) 1523-1528.
DOI URL |
[30] |
S. Chen, C. Li, T. Hou, Y. Cai, L. Liang, L. Chen, M. Li, React. Funct. Polym. 145 (2019), 104379.
DOI URL |
[31] |
W. Cao, D. Wei, Y. Jiang, S. Ye, A. Zheng, Y. Guan, J. Mater. Sci. 54 (2019) 2699-2711.
DOI |
[32] |
M.S. Brzezinska, M. Walczak, U. Jankiewicz, M. Pejchalova, J. Polym. Environ. 26 (2018) 589-595.
DOI URL |
[33] |
Y. Su, T. Feng, W. Feng, Y. Pei, Z. Li, J. Huo, C. Xie, X. Qu, P. Li, W. Huang, Macromol. Rapid Commun. 40 (2019), 1900268.
DOI URL |
[34] |
Q. Gao, T. Feng, D. Huang, P. Liu, P. Lin, Y. Wu, Z. Ye, J. Ji, P. Li, W. Huang, Biomater. Sci. 8 (2019) 278-289.
DOI URL |
[35] |
J. Peyre, V. Humblot, C. Methivier, J.M. Berjeaud, C.M. Pradier, J. Phys. Chem. B 116 (2012) 13839-13847.
DOI URL |
[36] |
Y. Su, Z. Zhi, Q. Gao, M. Xie, M. Yu, B. Lei, P. Li, P.X. Ma, Adv. Healthcare Mater. 6 (2017), 1601173.
DOI URL |
[37] |
Q. Wang, L. Wang, L. Gao, L. Yu, W. Feng, N. Liu, M. Xu, X. Li, P. Li, W. Huang, J. Mater. Chem. B 7 (2019) 3865-3875.
DOI URL |
[38] |
M. Xu, A. Khan, T. Wang, Q. Song, C. Han, Q. Wang, L. Gao, X. Huang, P. Li, W. Huang, ACS Appl. Bio Mater. 2 (2019) 3329-3340.
DOI URL |
[39] | D.C. Li, L.N. Zhang, Y. Zhu, M.Q. Li, Rare Met. Mater. Eng. 40 (2011) 87-90. |
[40] |
Y. Ha, J. Yang, F. Tao, Q. Wu, Y.J. Song, H.R. Wang, X. Zhang, P. Yang, Adv. Funct. Mater. 28 (2018), 1704476.
DOI URL |
[41] |
Y. Guan, H. Zhang, A. Zheng, D. Wei, J. Hu, Z. Wang, X. Xu, Polym. Adv. Technol. 30 (2019) 1555-1563.
DOI URL |
[42] |
H. Liu, R. Liu, I. Ullah, S. Zhang, Z. Sun, L. Ren, K. Yang, J. Mater, Sci. Technol. 48 (2020) 130-139.
DOI URL |
[43] | J.K. Zhang, W.H. Zhou, H. Wang, K.L. Lin, F.S. Chen, J. Mater, Sci. Technol. 35 (2019) 336-343. |
[44] |
R.B. Rodriguez, M.A. Regazzoni, J. Colloid Interface Sci. 177 (1996) 122-131.
DOI URL |
[45] |
N. Nakajima, Y. Ikada, Bioconjugate Chem. 6 (1995) 123-130.
PMID |
[46] |
Y. Yang, X. Li, H. Qiu, P. Li, P. Qi, M.F. Maitz, T. You, R. Shen, Z. Yang, W. Tian, N. Huang, ACS Appl. Mater. Interfaces 10 (2018) 7649-7660.
DOI URL |
[47] |
C. Zhao, G. Zhang, X. Xu, F. Yang, Y. Yang, Colloids Surf. A 512 (2017) 41-50.
DOI URL |
[48] |
S. Noel, B. Liberelle, L. Robitaille, G. De Crescenzo, Bioconjugate Chem. 22 (2011) 1690-1699.
DOI URL |
[49] |
J.H. Ryu, P.B. Messersmith, H. Lee, ACS Appl. Mater. Interfaces 10 (2018) 7523-7540.
DOI URL |
[50] | M.J. Lerman, J. Lembong, S. Muramoto, G. Gillen, J.P. Fisher, Tissue Eng. 24 (2018) 359-372. |
[51] |
S. Halder, K.K. Yadav, R. Sarkar, S. Mukherjee, P. Saha, S. Haldar, S. Karmakar, T. Sen, Springerplus 4 (2015) 672.
DOI PMID |
[52] |
L. Qian, Y. Guan, B. He, H. Xiao, Polymer 49 (2008) 2471-2475.
DOI URL |
[53] |
Z. Zhou, A. Zheng, J. Zhong, Acta Biochim. Biophys. Sin. 43 (2011) 729-737.
DOI URL |
[54] |
A. de Breij, M. Riool, P.H. Kwakman, L. de Boer, R.A. Cordfunke, J.W. Drijfhout, O. Cohen, N. Emanuel, S.A. Zaat, P.H. Nibbering, T.F. Moriarty, J. Control. Release 222 (2016) 1-8.
DOI PMID |
[55] | L. Damiati, M.G. Eales, A.H. Nobbs, B. Su, P.M. Tsimbouri, M. Salmeron-Sanchez, M.J. Dalby, J. Tissue Eng. 9 (2018) 1-16. |
[56] |
G. Lanzani, Nat. Mater. 13 (2014) 775-776.
DOI URL |
[57] |
W. Zhang, J. Liu, K. Yang, W. Qu, K. Liu, N. Liu, B. Gu, N. Hu, J. Ji, P.K. Chu, H. Wang, Adv. Mater. Interfaces 6 (2019), 1901414.
DOI URL |
[58] |
I. De Luca, A. Di Salle, N. Alessio, S. Margarucci, M. Simeone, U. Galderisi, A. Calarco, G. Peluso, Stem Cell Res. 17 (2016) 248-255.
DOI URL |
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