SERS-encoded nanocomposites for dual pathogen bioassay

doi:10.1016/j.jmst.2019.10.032

Abstract

Abstract:

Surface-enhanced Raman spectroscopy (SERS) has been successfully applied to detect various biomolecules, but it is still in challenge to assay living cells or bacteria sensitively, selectively and quantitatively in complex environments. In this paper, 4-ATP and DTNB are assembled on Ag nanoparticle (NP) -decorated poly (styrene-co-acrylic acid) (PSA) nanospheres and then sealed by silica shells to form sensitive SERS labels based on the localized surface plasmon resonance of Ag NPs and large light scattering cross-sections of PSA nanospheres. They are further developed as encoding tags for dual detection of S. aureus and E. coli after assembling corresponding aptamers, which demonstrate ultralow detection limits of 8 cell L^-1 for S. aureus and 2 cell L^-1 for E. coli. Such a bioassay indicates a point-of-care strategy of ultrasensitively biomedical detections by encoding specific SERS tags.

Key words: SERS, Pathogens, Dual detection, Encoded nanocomposites, Complex environments

Yaqi Shan, Mingliang Wang, Zengliang Shi, Milan Lei, Xiaoxuan Wang, Fu-Gen Wu, Huan-Huan Ran, Gowri Manohari Arumugam, Qiannan Cui, Chunxiang Xu. SERS-encoded nanocomposites for dual pathogen bioassay[J]. J. Mater. Sci. Technol., 2020, 43: 161-167.

Figures/Tables 10

Scheme 1. Schematic illustration of dual detection of S. aureus and E. coli based on SERS-encoded nanocomposites.

Fig. 1. Diagrammatic drawing for the preparation process of SERS probe (PSA/Ag NPs/4-ATP/SiO2 and PSA/Ag NPs/DTNB/SiO2).

Fig. 2. SEM images of (a) PSA, (b) PSA/Ag NPs, and (c) PSA/Ag NPs/SiO2; TEM images of (d) PSA, (e) PSA/Ag NPs, and (f) PSA/Ag NPs/SiO2; Size distribution curves of (g) PSA, (h) PSA/Ag NPs and (i) PSA/Ag NPs/SiO2.

Fig. 3. XRD patterns of PSA nanospheres, PSA/Ag NPs composites and PSA/Ag NPs/SiO2 composites.

Fig. 4. Absorption spectra of PSA nanospheres, PSA/Ag NPs composites and PSA/Ag NPs/SiO2 composites.

Fig. 5. FTIR spectra of PSA nanospheres, PSA/Ag NPs composites and PSA/Ag NPs/SiO2 composites.

Fig. 6. Raman spectra for 4-ATP, Ag NPs/4-ATP, PSA/Ag NPs/4-ATP/SiO2, PSA/Ag NPs/DTNB/SiO2, and mixtures of PSA/Ag NPs/4-ATP/SiO2 and PSA/Ag NPs/DTNB/SiO2.

Fig. 7. Schematic of the sensing scheme for simultaneous detection of S. aureus and E. coli based on SERS-encoded nanocomposites.

Fig. 8. (a) Raman spectra for S. aureus and E. coli with different concentrations which identified by PSA/Ag NPs/SiO2-Aptamer; (b) linear dependence of Raman peak intensity at 1436 cm-1 as a function of S. aureus concentrations (n = 5) and (c) linear dependence of Raman peak intensity at 1323 cm-1 as a function of E. coli concentrations (n = 5).

Fig. 9. Selectivity of the dual pathogen bioassays based on SERS-encoded nanocomposites.

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