Polysaccharide-based magnetically responsive polyelectrolyte hydrogels for tissue engineering applications

doi:10.1016/j.jmst.2017.10.003

Abstract

Abstract:

Polysaccharide-based bionanocomposite hydrogels with functional nanomaterials were used in biomedical applications. Self-organization of xanthan gum and chitosan in the presence of iron oxide magnetic nanoparticles (Fe₃O₄ MNPs) allowed us to form magnetically responsive polyelectrolyte complex hydrogels (MPECHs) via insitu ionic complexation using D-(+)-glucuronic acid δ-lactone as a green acidifying agent. Characterization confirmed the successful formation of (and structural interactions within) the MPECH and good porous structure. The rheological behavior and compressive properties of the PECH and MPECH were measured. The results indicated that the incorporation of Fe₃O₄ MNPs into the PECH greatly improved mechanical properties and storage modulus (G'). In vitro cell culture of NIH3T3 fibroblasts on MPECHs showed improvements in cell proliferation and adhesion in an external magnetic field relative to the pristine PECH. The results showed that the newly developed MPECH could potentially be used as a magnetically stimulated system in tissue engineering applications.

Key words: Magnetic nanoparticles, Bionanocomposite, Hydrogels, Cell proliferation, Adhesion, Tissue engineering

Kummara Madhusudana Rao, Anuj Kumar, Sung Soo Han. Polysaccharide-based magnetically responsive polyelectrolyte hydrogels for tissue engineering applications[J]. J. Mater. Sci. Technol., 2018, 34(8): 1371-1377.

Figures/Tables 7

Fig. 1. TEM images of Fe3O4 MNPs at (a) low magnification, (b) high magnification (c) their electron diffraction pattern, (d) digital images of MPECH, (e) digital images of MPECH attracted to magnetic bar, and (f) schematic representation of formation of MPECH.

Fig. 2. (A) FTIR spectra and (B) TGA curves of (a) CS, (b) XG, (c) PECH, (d) MPECH, and (e) Fe3O4 MNPs.

Fig. 3. XRD patterns of (a) XG, (b) PECH, (c) MPECH, and (d) Fe3O4 MNPs.

Fig. 4. (a) Frequency sweep rheology and (b) compressive stress-strain curves of the PECH and MPECH.

Fig. 5. SEM images of (a) PECH (low magnification), (a-1, a-2) PECH (high magnification) and (b) MPECH (low magnification), and (b-1, b-2) MPECH (high magnification).

Fig. 6. SEM images of NIH3T3 fibroblasts cell attachment on (a, a-1) PECH, (b, b-1) MPECH, and (c, c-1) MPECH with magnetic field for 1 day and 3 day of incubation.

Fig. 7. Proliferation of NIH3T3 cells cultured on PECH and MPECH with or without magnetic field.

References

[1]	Z. Rabia, M.Z. Khalid, T. Shazia, J. Farukh, N. Aqdas, Z. Mohammad, Int. J. Biol.Macromol. 92(2016) 1012-1024.
[2]	V. Karolis, N. Ben, G. Kees, M.M. Fokko, J.M.K. Ger, J.P. Stephen,Macromolecules 48 (2015) 8323-8330.
[3]	K. Madhusudana Rao, S. Nagappan, D.J. Seo, C.S. Ha, Appl. Clay Sci. 97(2014)33-42.
[4]	K. Madhusudana Rao, A. Kumar, S.S. Han, Int. J. Biol. Macromol. 101(2017)165-171.
[5]	A. Kumar, K. Madhusudana Rao, S.S. Han, Chem. Eng. J. 317(2017) 119-131.
[6]	M.A. Shamekhi, A. Rabiee, H. Mirzadeh, H. Mahdavi, D. Mohebbi-Kalhori, M.B.Eslaminejad, Mater. Sci. Eng. C 80 (2017) 532-542.
[7]	K. Kanimozhi, S. KhaleelBasha, V. Sugantha Kumari, Mater. Sci. Eng. C 61(2016) 484-491.
[8]	H.L. Kim, G.Y. Jung, J.H. Yoon, J.S. Han, Y.J. Park, D.G. Kim, M. Zhang, D.J. Kim,Mater. Sci. Eng. C 54 (2015) 20-25.
[9]	J. Shang, Z. Shao, X. Chen, Biomacromolecules 9 (2008) 1208-1213.
[10]	A.I. Gamzazade, S.M. Nasibov, Carbohydr. Polym. 50(2002) 339-343.
[11]	D.A. Maciel, H.C.B.Paula, R.C.M.De Paula, Eur. Polym. J. 41(2005) 2726-2733.
[12]	P. Dong, W. Hao, Y. Xia, G. Da, T. Wang, J. Mater.Sci.Technol. 26(2010)1027-1031.
[13]	H.J. Kwon, K. Yasuda, J.P. Gong, Y. Ohmiya, Macromol. Res. 22(2014) 227-235.
[14]	D. Magnin, J. Lefebvre, E. Chornet, S. Dumitriu, Carbohydr. Polym. 55(2004)437-453.
[15]	N. Almeida, A. Mueller, S. Hirschi, L. Rakesh, J. Biomed. Mater. Res. A 102(2014) 1510-1517.
[16]	M.Z. Bellini, C. Caliari-Oliveira, A. Mizukami, K. Swiech, D.T. Covas, E.A.Donadi, P. Oliva-Neto, A.M. Moraes, J. Biomater. 29(2015) 1155-1166.
[17]	V.B. Bueno, S.H. Takahashi, L.H. Catalani, S. Torresi, D.F. Siqueira Petri, Mater.Sci. Eng. C . 52 (2015) 121-128.
[18]	A. Kumar, K. Madhusudana Rao, S.E. Kwon, Y.N. Lee, S.S. Han, Mater. Lett. 193(2017) 274-278.
[19]	K. Madhusudana Rao, A. Kumar, S.S. Han, Int. J. Biol. Macromol. 101(2017)165-171.
[20]	Y. Shchipunov, S. Sarin, K. IL, C.S. Ha, Green Chem. 12(2010) 1187-1195.
[21]	J.O. Fergal, Mater. Today 14 (2011) 88-95.
[22]	G. Sara, M.S. Joana, F.M. Joao, ACS Biomater. Sci. Eng. 1(2015) 1016-1025.
[23]	N. Zhang, J. Lock, A. Sallee, H. Liu, ACS Appl. Mater. Interfaces 7 (2015)20987-20998.
[24]	A. Pourjavadi, S.H. Hosseini, M. Doulabi, S.M. Fakoorpoor, F. Seidi, ACS Catal. 2(2012) 1259-1266.
[25]	C. Hui, C. Shen, T. Yang, L. Bao, J. Tian, H. Ding, C. Li, H.J. Gao, J. Phys. Chem. C112(2008) 11336-11339.
[26]	K.S.V.Krishna Rao, K.Madhusudana Rao, P.R. Reddy, N.S. Reddy, S. Yury, C.S.Ha,Polym. Int. 64(2015) 393-402.
[27]	P.M.C.Marcia, L.M.F.Ivana, T.M.C. Mauricio,Carbohydr. Polym. 146(2016)123-130.
[28]	T. Pan, W. Song, X. Cao, Y. Wang, J. Mater.Sci.Technol. 32(2016) 889-900.
[29]	M. Ebrahimian-Hosseinabadi, F. Ashrafizadeh, M. Etemadifar, S.S.Venkatraman, J. Mater.Sci.Technol. 27(2011) 1105-1112.