J. Mater. Sci. Technol. ›› 2016, Vol. 32 ›› Issue (9): 944-949.DOI: 10.1016/j.jmst.2016.08.009
• Orginal Article • Previous Articles Next Articles
Xu Shao1,Zhou Zhiyu2,Gao Manman2,Zou Changye3,Che Yinglin1,Cody Bünger4,Zou Xuenong2,*(),Zhou Lei5,**()
Received:
2015-11-30
Accepted:
2016-04-08
Online:
2016-09-20
Published:
2016-11-02
Contact:
Zou Xuenong,Zhou Lei
Xu Shao,Zhou Zhiyu,Gao Manman,Zou Changye,Che Yinglin,Cody Bünger,Zou Xuenong,Zhou Lei. Bioadaptive Nanorod Topography of Titanium Surface to Control Cell Behaviors and Osteogenic Differentiation of Preosteoblast Cells[J]. J. Mater. Sci. Technol., 2016, 32(9): 944-949.
Gene symbol | Forward primer | Reversed primer |
---|---|---|
GAPDH | CTTGGGCTACACTGAGGACC | CATACCAGGAAATGAGCTTGAC |
ALP | TGGAAGGAGGCAGGATTGA | ATCAGCAGTAACCACAGTCA |
OCN | CAGACAAGTCCCACACAG | GTGAGGTCAGAGAGACAG |
RUNX2 | AATGCCTCCGCTGTTATGA | TTGTGAAGACTGTTATGGTCAAG |
COL1A1 | CCTGGCAAAGACGGACTCA | GGGCTGCGGATGTTCTCAAT |
Osterix | TCTCAAGCACCAATGGACT | AGGAAATGAGTGAGGGAAGG |
Table 1 Primer sequences of genes
Gene symbol | Forward primer | Reversed primer |
---|---|---|
GAPDH | CTTGGGCTACACTGAGGACC | CATACCAGGAAATGAGCTTGAC |
ALP | TGGAAGGAGGCAGGATTGA | ATCAGCAGTAACCACAGTCA |
OCN | CAGACAAGTCCCACACAG | GTGAGGTCAGAGAGACAG |
RUNX2 | AATGCCTCCGCTGTTATGA | TTGTGAAGACTGTTATGGTCAAG |
COL1A1 | CCTGGCAAAGACGGACTCA | GGGCTGCGGATGTTCTCAAT |
Osterix | TCTCAAGCACCAATGGACT | AGGAAATGAGTGAGGGAAGG |
Fig. 1. Microscopic images of untreated and titanium nanorods surface with and without cellular adhesion of preosteoblast MC3T3-E1. SEM images of untreated titanium surface (A), nanorods titanium surface (B), SEM images of MC3T3-E1 cultured on untreated titanium surface (C) and on the titanium nanorods surface (D) after 24?h of incubation. Fluorescence microscopy images of cytoskeletal actin (green) and nucleus-staining DAPI (blue) for MC3T3-E1 cells cultured on untreated titanium surface (E) and on the titanium nanorods surface (F) after 24?h of culture incubation.
Fig. 2. ALP activity of preosteoblast MC3T3-E1 detected after 7, 14 and 21 days of incubation on untreated titanium and titanium nanorods surface. Con: untreated titanium, Ti: titanium nanorods. *p?<?0.05, **p?<?0.01.
Fig. 3. Osteogenic differentiation markers expression analysis of preosteoblast MC3T3-E1 cultured on untreated titanium and titanium nanorods surface after 7, 14 and 21 days of incubation using real-time PCR: (A) ALP, (B) OCN, (C) COL1A1. Con: untreated titanium, Ti: titanium nanorods. *p?<?0.05, **p?<?0.01.
Fig. 4. Runx2 expression analysis of preosteoblast MC3T3-E1 cultured on untreated titanium and titanium nanorods surface after 7, 14 and 21 days of incubation using real-time PCR. *p?<?0.05.
Fig. 5. Osterix expression analysis of preosteoblast MC3T3-E1 cultured on untreated titanium and titanium nanorods surface after 7, 14 and 21 days of incubation with real-time PCR (A) and with western-blot (B). *p?<?0.05.
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