J. Mater. Sci. Technol. ›› 2016, Vol. 32 ›› Issue (9): 950-955.DOI: 10.1016/j.jmst.2016.06.023
• Orginal Article • Previous Articles Next Articles
Zhou Zhengnan1,2,Li Weiping1,2,He Tianrui1,2,Yu Peng1,2,Tan Guoxin3,*(),Ning Chengyun1,2,**()
Received:
2015-09-30
Accepted:
2015-12-02
Online:
2016-09-20
Published:
2016-11-02
Contact:
Tan Guoxin,Ning Chengyun
Zhou Zhengnan,Li Weiping,He Tianrui,Yu Peng,Tan Guoxin,Ning Chengyun. Controllable Protein Adsorption and Bacterial Adhesion on Polypyrrole Nanocone Arrays[J]. J. Mater. Sci. Technol., 2016, 32(9): 950-955.
Fig. 2. FE-SEM images of PPy-NSA nanocone arrays in three states: (a) original state, (c) reduction state, (d) oxidation state. (b) The cyclic voltammetry curve of PPy-NSA nanocone arrays. The results showed that the PPy-NSA nanocone arrays were significant redox, the Eox?=?-0.24?V, Ere?= ?-0.48?V. From the cyclic voltammetry curve, -0.8?V and -0.15?V were chosen as reduction potential and oxidation potential, respectively, for making reduction state and oxidation state PPy-NSA nanocone arrays. In reduction state, PPy-NSA nanocones were closed, while in oxidation state, they were open.
Fig. 3. SKPM surface potential imaging patterns of PPy-NSA nanocone arrays in three states: (a) original state, (b) reduction state, (c) oxidation state. (d) Zeta potential of PPy-NSA nanocone arrays in three states. (e) Contact angles of PPy-NSA nanocone arrays in three states.
Fig. 4. EPMA profiles of the PPy-NSA nanocone arrays in three states. The results showed that the chemical composition of PPy-NSA nanocone arrays in three states was different. It suggested that NSA as counterions were doped into and removed from the PPy-NSA nanocone arrays during the redox reaction.
Fig. 5. Protein adsorption results of the PPy-NSA nanocone arrays in three states. (a) Standard curve of BSA in the BCA protein assay. (b) The amount of adsorbed BSA on PPy-NSA nanocone arrays.Notes: N?=?3, *p?<?0.05, **p?<?0.01, the original state as control group.
Fig. 6. Plate bacterial colony count images of PPy-NSA nanocone arrays in three states co-cultured with S. aureus for 4?h and after 24?h of incubation: (a) reduction state, (b) original state, (c) oxidation state. (d) Bar graph of the adhered bacterial colonies on three states of PPy-NSA nanocone arrays. (e) Sterilizing rate of S. aureus on PPy-NSA nanocone arrays in three states.Notes: (1) values are averages with standard deviations of at least 4 pictures conducted on a minimum of two separate experiments. (2) N?=?4, *p?<?0.05, **p?<?0.01, the original state as control group.
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