Properties and Cytocompatibility of Anti-Washout Calcium Phosphate Cement by Introducing Locust Bean Gum

doi:10.1016/j.jmst.2016.05.011

Abstract

Abstract:

The washout resistance of injectable calcium phosphate cement (CPC) is highly requisite for more widely clinical applications. In this work, locust bean gum (LBG) was used as the anti-washout agent to improve the washout resistance of CPC. The results indicated that the washout resistance was greatly improved, and meanwhile the injectability, setting time and compressive strength slightly decreased when the content of LBG was no more than 1.0%. Additionally, the CPC with 1.0% LBG exhibited good cell compatibility of the mouse bone mesenchymal stem cells (mBMSCs). Therefore, the 1.0% LBG content was proposed to serve as a useful additive in CPC as a result of its ability to promote washout resistance, which may play an important role in clinical applications.

Key words: Calcium phosphate cement, Locust bean gum, Washout resistance, Cytocompatibility

Liu Jingqun,Li Jiyan,Ye Jiandong. Properties and Cytocompatibility of Anti-Washout Calcium Phosphate Cement by Introducing Locust Bean Gum[J]. J. Mater. Sci. Technol., 2016, 32(10): 1021-1026.

Figures/Tables 7

Fig. 1. FTIR spectra of LBG.

Fig. 2. Influence of the content of LBG on the washout resistance (a, b) and injectability (c) of the cement pastes.

Fig. 3. Influence of the content of LBG on the yield stress (a) and the viscosity (b) of the cement pastes.

Fig. 4. Influence of the content of LBG on the setting time (a) and compressive strength (b) of CPC.

Fig. 5. XRD patterns (a) and SEM images (b) of the hydrated cements with 1.0% LBG and without LBG.

Fig. 6. SEM images and fluorescence photos of mBMSCs cultured on the cements with 1.0% LBG and without LBG after 1 and 3 days.

Fig. 7. mBMSCs proliferation (a) on the cements with 1.0% LBG and without LBG after being cultured for 1, 3, and 7 days and ALP activity (b) of mBMSCs cultured on the cements after 7 and 10 days.

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