J. Mater. Sci. Technol. ›› 2018, Vol. 34 ›› Issue (12): 2359-2367.DOI: 10.1016/j.jmst.2018.06.011
Special Issue: Nanomaterials 2018; Composites 2018
• Orginal Article • Previous Articles Next Articles
Dunhua Hongabc, Guangzhong Caoa*(), Junle Qub, Yuanming Dengc*(), Jiaonin Tangc
Received:
2017-07-18
Revised:
2017-08-27
Accepted:
2017-09-25
Online:
2018-12-20
Published:
2018-11-15
Contact:
Cao Guangzhong,Deng Yuanming
Dunhua Hong, Guangzhong Cao, Junle Qu, Yuanming Deng, Jiaonin Tang. Antibacterial activity of Cu2O and Ag co-modified rice grains-like ZnO nanocomposites[J]. J. Mater. Sci. Technol., 2018, 34(12): 2359-2367.
Cu2O-Ag/ZnO | Cu2O/ZnO | Ag/ZnO | ||||||
---|---|---|---|---|---|---|---|---|
Samples | Compositions | Samples | Compositions | Samples | Compositions | |||
Design | Products (EDS) | Design | Products(EDS) | Design | Products(EDS) | |||
S1 | (Cu2O)0.02Ag0.08ZnO0.9 | Cu0.39Ag0.079Zn0.9O0.92 | K0(control) | ZnO | L1 | Ag0.02ZnO0.98 | AgNDZn0.98O0.98 | |
S2 | (Cu2O)0.04Ag0.06ZnO0.9 | Cu0.04Ag0.06Zn0.9O0.935 | K1 | (Cu2O)0.02ZnO0.98 | Cu0.032Zn0.98O0.993 | L2 | Ag0.04ZnO0.96 | Ag0.036Zn0.96O0.96 |
S3 | (Cu2O)0.06Ag0.04ZnO0.9 | Cu0.06Ag0.038Zn0.9O0.962 | K2 | (Cu2O)0.04ZnO0.96 | Cu0.076Zn0.96O1.01 | L3 | Ag0.06ZnO0.94 | Ag0.062Zn0.94O0.942 |
S4 | (Cu2O)0.08Ag0.02ZnO0.9 | Cu0.08AgNDZn0.9O0.979 | K3 | (Cu2O)0.06ZnO0.94 | Cu0.117Zn0.94O0.997 | L4 | Ag0.08ZnO0.92 | Ag0.086Zn0.92O0.918 |
K4 | (Cu2O)0.08ZnO0.92 | Cu0.152Zn0.92O0.99 | L5 | Ag0.1ZnO0.9 | Ag0.098Zn0.9O0.903 | |||
K5(control) | Cu2O |
Table 1 Compositions and sample codes of Cu2O-Ag/ZnO, Cu2O/ZnO and Ag/ZnO.
Cu2O-Ag/ZnO | Cu2O/ZnO | Ag/ZnO | ||||||
---|---|---|---|---|---|---|---|---|
Samples | Compositions | Samples | Compositions | Samples | Compositions | |||
Design | Products (EDS) | Design | Products(EDS) | Design | Products(EDS) | |||
S1 | (Cu2O)0.02Ag0.08ZnO0.9 | Cu0.39Ag0.079Zn0.9O0.92 | K0(control) | ZnO | L1 | Ag0.02ZnO0.98 | AgNDZn0.98O0.98 | |
S2 | (Cu2O)0.04Ag0.06ZnO0.9 | Cu0.04Ag0.06Zn0.9O0.935 | K1 | (Cu2O)0.02ZnO0.98 | Cu0.032Zn0.98O0.993 | L2 | Ag0.04ZnO0.96 | Ag0.036Zn0.96O0.96 |
S3 | (Cu2O)0.06Ag0.04ZnO0.9 | Cu0.06Ag0.038Zn0.9O0.962 | K2 | (Cu2O)0.04ZnO0.96 | Cu0.076Zn0.96O1.01 | L3 | Ag0.06ZnO0.94 | Ag0.062Zn0.94O0.942 |
S4 | (Cu2O)0.08Ag0.02ZnO0.9 | Cu0.08AgNDZn0.9O0.979 | K3 | (Cu2O)0.06ZnO0.94 | Cu0.117Zn0.94O0.997 | L4 | Ag0.08ZnO0.92 | Ag0.086Zn0.92O0.918 |
K4 | (Cu2O)0.08ZnO0.92 | Cu0.152Zn0.92O0.99 | L5 | Ag0.1ZnO0.9 | Ag0.098Zn0.9O0.903 | |||
K5(control) | Cu2O |
Fig. 3. (a) and (b) Low resolution TEM images of sample S2, (c) HRTEM images of ZnO and Ag nanoparticles, and (d) HRTEM images of ZnO and Cu2O nanoparticles.
Fig. 4. Surface particles size distribution histogram of synthesized (Cu2O)0.04Ag0.06ZnO0.9 antimicrobial agent: (a) ZnO nanoparticles, (b) Cu2O nanoparticles, and (c) Ag nanoparticles.
Fig. 5. (a) Wide XPS spectrum of sample S2. The magnified XPS spectra corresponding to the individual elements of (b) zinc, (c) copper, and (d) silver.
Concentration of samples [μg/mL] | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
2000 | 1000 | 500 | 250 | 125 | 62.5 | 31.25 | 15.63 | 7.82 | 3.91 | |
S1 | - | - | - | - | - | - | ++ | ++ | ++ | ++ |
S2 | - | - | - | - | - | - | - | ++ | ++ | ++ |
S3 | - | - | - | - | - | + | ++ | ++ | ++ | ++ |
S4 | - | - | - | - | ++ | ++ | ++ | ++ | ++ | ++ |
K0 | - | + | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ |
K1 | - | - | + | ++ | + | + | ++ | ++ | ++ | ++ |
K2 | - | - | - | + | ++ | ++ | ++ | ++ | ++ | ++ |
K3 | - | - | - | - | - | ++ | ++ | ++ | ++ | ++ |
K4 | - | - | - | - | ++ | ++ | ++ | ++ | ++ | ++ |
K5 | - | - | - | - | - | ++ | ++ | ++ | ++ | ++ |
L1 | - | - | + | ++ | ++ | ++ | ++ | ++ | ++ | ++ |
L2 | - | - | - | - | + | ++ | ++ | ++ | ++ | ++ |
L3 | - | - | - | - | + | ++ | ++ | ++ | ++ | ++ |
L4 | - | - | - | - | - | + | ++ | ++ | ++ | ++ |
L5 | - | - | - | - | - | + | ++ | ++ | ++ | ++ |
Table 2 MIC measurements of all samples against E. coli.
Concentration of samples [μg/mL] | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
2000 | 1000 | 500 | 250 | 125 | 62.5 | 31.25 | 15.63 | 7.82 | 3.91 | |
S1 | - | - | - | - | - | - | ++ | ++ | ++ | ++ |
S2 | - | - | - | - | - | - | - | ++ | ++ | ++ |
S3 | - | - | - | - | - | + | ++ | ++ | ++ | ++ |
S4 | - | - | - | - | ++ | ++ | ++ | ++ | ++ | ++ |
K0 | - | + | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ |
K1 | - | - | + | ++ | + | + | ++ | ++ | ++ | ++ |
K2 | - | - | - | + | ++ | ++ | ++ | ++ | ++ | ++ |
K3 | - | - | - | - | - | ++ | ++ | ++ | ++ | ++ |
K4 | - | - | - | - | ++ | ++ | ++ | ++ | ++ | ++ |
K5 | - | - | - | - | - | ++ | ++ | ++ | ++ | ++ |
L1 | - | - | + | ++ | ++ | ++ | ++ | ++ | ++ | ++ |
L2 | - | - | - | - | + | ++ | ++ | ++ | ++ | ++ |
L3 | - | - | - | - | + | ++ | ++ | ++ | ++ | ++ |
L4 | - | - | - | - | - | + | ++ | ++ | ++ | ++ |
L5 | - | - | - | - | - | + | ++ | ++ | ++ | ++ |
Concentration of samples [μg/mL] | ||||||||
---|---|---|---|---|---|---|---|---|
2000 | 1000 | 500 | 250 | 125 | 62.5 | 31.25 | 15.63 | |
S1 | - | - | - | - | + | ++ | ++ | ++ |
S2 | - | - | - | - | - | ++ | ++ | ++ |
S3 | - | - | - | - | + | + | ++ | ++ |
S4 | - | - | - | - | + | ++ | ++ | ++ |
K0 | - | ++ | ++ | ++ | ++ | ++ | ++ | ++ |
K1 | - | - | ++ | ++ | ++ | ++ | ++ | ++ |
K2 | - | - | + | ++ | ++ | ++ | ++ | ++ |
K3 | - | - | - | - | ++ | ++ | ++ | ++ |
K4 | - | - | - | + | ++ | ++ | ++ | ++ |
L1 | - | - | + | ++ | ++ | ++ | ++ | ++ |
L2 | - | - | - | + | ++ | ++ | ++ | ++ |
L3 | - | - | - | - | ++ | ++ | ++ | ++ |
L4 | - | - | - | - | ++ | ++ | ++ | ++ |
L5 | - | - | - | ++ | ++ | ++ | ++ | ++ |
Table 3 MIC measurements of all samples against S. aureus.
Concentration of samples [μg/mL] | ||||||||
---|---|---|---|---|---|---|---|---|
2000 | 1000 | 500 | 250 | 125 | 62.5 | 31.25 | 15.63 | |
S1 | - | - | - | - | + | ++ | ++ | ++ |
S2 | - | - | - | - | - | ++ | ++ | ++ |
S3 | - | - | - | - | + | + | ++ | ++ |
S4 | - | - | - | - | + | ++ | ++ | ++ |
K0 | - | ++ | ++ | ++ | ++ | ++ | ++ | ++ |
K1 | - | - | ++ | ++ | ++ | ++ | ++ | ++ |
K2 | - | - | + | ++ | ++ | ++ | ++ | ++ |
K3 | - | - | - | - | ++ | ++ | ++ | ++ |
K4 | - | - | - | + | ++ | ++ | ++ | ++ |
L1 | - | - | + | ++ | ++ | ++ | ++ | ++ |
L2 | - | - | - | + | ++ | ++ | ++ | ++ |
L3 | - | - | - | - | ++ | ++ | ++ | ++ |
L4 | - | - | - | - | ++ | ++ | ++ | ++ |
L5 | - | - | - | ++ | ++ | ++ | ++ | ++ |
Samples | Morphology | Particle size (nm) | bacterial species | MIC (μg/mL) | MBC (μg/mL) | Ref. |
---|---|---|---|---|---|---|
(Cu2O)0.04Ag0.06ZnO0.9 | nanoparticles | ZnO: 202?nm, Ag: 30?nm, Cu2O: 30?nm | E. coli S. aureus | 31.25 125 | 250 375 | This work |
Cu2O(or Cu2O-AA) | nanoparticles | 50?nm, 260?nm, 340 nm | E. coli | 100, 150, 300 | - | [ |
Ag/ZnO-activated carbon | nanoparticles | 30-45?nm | E. coli S. aureus | 200 300 | 350 600 | [ |
Ag/ZnO [Zn:Ag (1:0.88)] | nanoparticles | 53 nm | GFP E. coli S. aureus | 550 60 | 600 70 | [ |
Ag/ZnO | nanoparticles | 500 nm | E. coli, | 200 (inhibition%?=?91%) | - | [ |
Ag0.02Zn0.98O0.99 | nanoparticles | 30?nm | E. coli S. aureus Candida albicans | 100 100 50 | 250 200 100 | [ |
Cu2O/Cu(orCu2O/Cu-Alg) | nanoparticles | 3-8 nm | E. coli S. aureus Salmonella typhimurium | 100 80 80 | 100 100 100 | [ |
Cu2O-natural clinoptilolite | nanoparticles | 50?nm | E. coli S. aureus | 10000 10000 | - - | [ |
ZnO | nanoparticles | 60 nm | E. coli S. aureus Pseudomonas aeruginosa Bacillus subtilis | 100 390 50 20 | 12500 12500 100 390 | [ |
Ag/ZnO | nanoparticles | 12 nm | E. coli S. aureus | 32 - | 512 - | [ |
ALE-ZnO | nanoparticles | 15?nm | E. coli S. aureus P. aeruginosa | 2000 2200 2000 | 2200 2800 2200 | [ |
ZnO(orGO@CS/ZnO) | nanoparticles | 30?nm | E. coli S. aureus | 100(or2.5) 100(or5) | - - | [ |
Table 4 Comparison of the antibacterial activity between the present one with previous reports.
Samples | Morphology | Particle size (nm) | bacterial species | MIC (μg/mL) | MBC (μg/mL) | Ref. |
---|---|---|---|---|---|---|
(Cu2O)0.04Ag0.06ZnO0.9 | nanoparticles | ZnO: 202?nm, Ag: 30?nm, Cu2O: 30?nm | E. coli S. aureus | 31.25 125 | 250 375 | This work |
Cu2O(or Cu2O-AA) | nanoparticles | 50?nm, 260?nm, 340 nm | E. coli | 100, 150, 300 | - | [ |
Ag/ZnO-activated carbon | nanoparticles | 30-45?nm | E. coli S. aureus | 200 300 | 350 600 | [ |
Ag/ZnO [Zn:Ag (1:0.88)] | nanoparticles | 53 nm | GFP E. coli S. aureus | 550 60 | 600 70 | [ |
Ag/ZnO | nanoparticles | 500 nm | E. coli, | 200 (inhibition%?=?91%) | - | [ |
Ag0.02Zn0.98O0.99 | nanoparticles | 30?nm | E. coli S. aureus Candida albicans | 100 100 50 | 250 200 100 | [ |
Cu2O/Cu(orCu2O/Cu-Alg) | nanoparticles | 3-8 nm | E. coli S. aureus Salmonella typhimurium | 100 80 80 | 100 100 100 | [ |
Cu2O-natural clinoptilolite | nanoparticles | 50?nm | E. coli S. aureus | 10000 10000 | - - | [ |
ZnO | nanoparticles | 60 nm | E. coli S. aureus Pseudomonas aeruginosa Bacillus subtilis | 100 390 50 20 | 12500 12500 100 390 | [ |
Ag/ZnO | nanoparticles | 12 nm | E. coli S. aureus | 32 - | 512 - | [ |
ALE-ZnO | nanoparticles | 15?nm | E. coli S. aureus P. aeruginosa | 2000 2200 2000 | 2200 2800 2200 | [ |
ZnO(orGO@CS/ZnO) | nanoparticles | 30?nm | E. coli S. aureus | 100(or2.5) 100(or5) | - - | [ |
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