J. Mater. Sci. Technol. ›› 2019, Vol. 35 ›› Issue (5): 817-823.DOI: 10.1016/j.jmst.2018.11.009

• Orginal Article • Previous Articles     Next Articles

Surface energy-mediated fibronectin adsorption and osteoblast responses on nanostructured diamond

Yixing Tiana, Huiling Liua, Brian W. Sheldonb, Thomas J. Webstercd, Sichen Yange, Huilin Yangad, Lei Yangad?()   

  1. aOrthopaedic Institute and Department of Orthopaedics, The First Affiliated Hospital, Soochow University, Suzhou, Jiangsu, 215006, China
    bSchool of Engineering, Brown University, Providence, RI, 02912, USA
    cDepartment of Chemical Engineering, Northeastern University, Boston, MA, 02115, USA
    dInternational Research Center for Translational Orthopaedics (IRCTO), Soochow University, Suzhou, Jiangsu, 215006, China
    eUWC Changshu China, Changshu, Jiangsu, 215500, China
  • Received:2018-08-02 Accepted:2018-10-17 Online:2019-05-10 Published:2019-02-20
  • Contact: Yang Lei
  • About author:

    1 These authors contribute equally to this paper.

Abstract:

Nanostructured diamond have potential applications in many biomedical related fields and demonstrated extraordinary capacity to influence cellular responses. Studying the surface property of nanodiamond and its influence to protein adsorption and subsequent cellular responses along with the mechanism behind such capacity becomes more important. Here the role of surface energy associated with nanostructured diamond in modulating fibronectin and osteoblast (OB, bone forming cells) responses was investigated. Nanocrystalline diamond (NCD) and submicron crystalline diamond (SMCD) films with controllable surface energy were prepared by microwave-enhanced plasma chemical vapor deposition (MPCVD) techniques. Fibronectin adsorption on the diamond films with varied surface energy values was measured via the enzyme-linked immunosorbent assay (ELISA) and the relationship between the surface energy and fibronectin adsorption was studied. The result indicated that fibronectin adsorption on nanostructured surfaces was closely related to both surface energy and material microstructures. The spreading and migration of OB aggregates (each containing 30-50 cells) on the NCD with varied surface energy values were also studied. The result indicates a correlation between the cell spreading and migration on nanodiamond and the surface energy of nanostructured surface.

Key words: Nanostructure, Diamond films, Surface characteristics, Protein adsorption, Cell responses