J. Mater. Sci. Technol. ›› 2016, Vol. 32 ›› Issue (8): 721-728.DOI: 10.1016/j.jmst.2016.05.009

• Orginal Article • Previous Articles     Next Articles

Antibacterial Action of Chemically Synthesized and Laser Generated Silver Nanoparticles against Human Pathogenic Bacteria

Zafar Nosheen1,Shamaila Shahzadi1,*(),Nazir Jawad2,Sharif Rehana1,Shahid Rafique Muhammad1,Ul-Hasan Jalees1,Ammara Syeda1,3,Khalid Hina1   

  1. 1 Department of Physics, University of Engineering and Technology, Lahore 54890, Pakistan
    2 Department of Microbiology, University of Veterinary and Animal Sciences, Lahore, Pakistan
    3 Department of Physics, Forman Christian College, A Charted University, Lahore, Pakistan
  • Received:2015-06-05 Accepted:2015-11-27 Online:2016-08-10 Published:2016-10-10
  • Contact: Shamaila Shahzadi

Abstract:

Silver nanoparticles in the range of 10-40?nm were synthesized chemically and by laser ablation, employed for in vitro antibacterial action against human pathogenic bacterium. Their formation was evidenced by UV-visible spectrophotometer; particle size confirmed by atomic force microscopy, crystal structure determined by X-ray diffraction and chemical composition investigated by Fourier transform infrared spectroscopy. The calculated MIC (minimum inhibitory concentration) of chemically synthesized nanoparticles with 30-40?nm in size are 2.8?µg/mL, 4.37?µg/mL, 13.5?µg/mL and 2.81?µg/mL for E. coli, S. aureus, B. subtillis and Salmonella, respectively. Whereas laser ablated nanoparticles exhibit MIC of 2.10?µg/mL, 2.36?µg/mL and 2.68?µg/mL for E. coli, S. aureus and Salmonella, respectively.

Key words: Chemically synthesized and laser ablated nanoparticles, UV-visible spectrophotometer, Gram negative and gram positive bacteria