Effect of Heat Treatment on Cu Distribution, Antibacterial Performance and Cytotoxicity of Ti-6Al-4V-5Cu Alloy

doi:10.1016/j.jmst.2015.04.002

Abstract

Abstract: A copper-bearing Ti-6Al-4V-5Cu alloy was processed and subjected to different heat treatments to explore the relationship among microstructure, antibacterial performance, and cytocompatibility. Characterization of microstructure revealed that the solution treated alloy consisted of α phase, α′ phase and β phase, while besides these phases, the aged alloy also contained the precipitations of intermetallic Ti₂Cu compound. The solution treated alloy showed better antibacterial performance with increasing the solution temperature. The Cu ions released from Ti-6Al-4V-5Cu alloy could effectively inhibit the formation of bacterial biofilm on the surface of alloy, and do not induce any cytotoxicity. The optimal heat treatment for Ti-6Al-4V-5Cu alloy was solution treated at 930 °C, at which it could exhibit both promising antibacterial performance and no cytotoxicity.

Key words: Ti-6Al-4V-5Cu, Heat treatment, Microstructure, Antibacterial performance, Cytocompatibility

Zheng Ma, Ling Ren, Rui Liu, Ke Yang, Yu Zhang, Zhenhua Liao, Weiqiang Liu, Min Qi, R.D.K. Misra. Effect of Heat Treatment on Cu Distribution, Antibacterial Performance and Cytotoxicity of Ti-6Al-4V-5Cu Alloy[J]. J. Mater. Sci. Technol., 2015, 31(7): 723-732.

Figures/Tables 10

Microstructures of Ti-6Al-4V-5Cu alloy under different heat treatments: (a) S-810, (b) S-930, (c) S-1050, (d) SA-810, (e) SA-930 and (f) SA-1050.

XRD patterns of Ti-6Al-4V-5Cu alloy under different heat treatments: (a) S-810, S-930 and S-1050 sample, (b) SA-810, SA-930 and SA-1050.

SEM observation and EDS analysis on SA-1050 sample: (a) SEM micrograph showing special morphology, (b) line scan of Cu across α

and β

phases, (c) comparison of elemental content in α

and β

phases obtained from two selected black squares highlighted in (a).*

p <

0.05.

TEM micrographs of S-930 sample (a) and SA-930 sample (b-d): (a) bright field image and the corresponding selected area diffraction pattern (SADP) along zone axis of [110]β

References

[1] K. Wang, Mater. Sci. Eng. A , 213 (1996), pp. 134-137
[2] A. Ewald, S.K. Gluckermann, R. Thull, U. Gbureck, Biomed. Eng. Online , 5 (2006), pp. 1-10
[3] J.G.E. Hendriks, J.R. van Horn, H.C. van der Mei, H.J. Busscher, Biomaterials , 25 (2004), pp. 545-556
[4] L.G. Harris, L. Mead, E. Müller-Oberländer, R.G. Richards, J. Biomed. Mater. Res. Part A , 78 (2006), pp. 50-58
[5] M. Yoshinari, Y. Oda, T. Kato, K. Okuda, Biomaterials , 22 (2001), pp. 2043-2048
[6] J.S. Kawalec, S.A. Brown, J.H. Payer, K. Merritt, J. Biomed. Mater. Res. , 29 (1995), pp. 867-873
[7] E.M. Hetrick, M.H. Schoenfisch, Chem. Soc. Rev. , 35 (2006), pp. 780-789
[8] P. Anguita-Alonso, A.D. Hanssen, R. Patel, Expert Rev. Anti Infect. Ther. , 3 (2005), pp. 797-804
[9] H. Hu, W. Zhang, Y. Qiao, X. Jiang, X. Liu, C. Ding, Acta Biomater. , 8 (2012), pp. 904-915
[10] L.Z. Zhao, P.K. Chu, Y.M. Zhang, Z.F. Wu, J. Biomed. Mater. Res. B , 91 (2009), pp. 470-480
[11] J.A. Liao, Z.M. Zhu, A.C. Mo, L. Li, J.C. Zhang, Int. J. Nanomed. , 5 (2010), pp. 261-267
[12] L. Song, Y.F. Xiao, L. Gan, Y. Wu, F. Wu, Z.W. Gu, Surf. Coat. Technol. , 206 (2012), pp. 2986-2990
[13] B.S. Necula, L.E. Fratila-Apachitei, S.A. Zaat, I. Apachitei, J. Duszczyk, Acta Biomater. , 5 (2009), pp. 3573-3580
[14] Y.F. Zheng, B.B. Zhang, B.L. Wang, Y.B. Wang, L. Li, Q.B. Yang, L.S. Cui, Acta Biomater. , 7 (2011), pp. 2758-2767
[15] K. Das, S. Bose, A. Bandyopadhyay, B. Karandikar, B.L. Gibbins, J. Biomed. Mater. Res. Part B , 87 (2008), pp. 455-460
[16] Y. Takeshi, T. Misako, O. Masayuki, Silver Dispersed Stainless Steel with Antibacterial Property, Kawasaki Steel Corporation, Tokyo, Japan (2002)
[17] L. Nan, Y. Liu, M. Lü, K. Yang, J. Mater. Sci. Mater. Med. , 19 (2008), pp. 3057-3062
[18] A. Devaraj, S. Nag, B.C. Muddle, R. Banerjee, Metall. Mater. Trans. A , 42 (2011), pp. 1139-1143
[19] X. Yao, Q.Y. Sun, L. Xiao, J. Sun, J. Alloy. Compd. , 484 (2009), pp. 196-202
[20] W.R. Osório, A. Cremasco, P.N. Andrade, A. Garcia, R. Caram, Electrochim. Acta , 55 (2010), pp. 759-770
[21] T. Okabe, M. Kikuchi, C. Ohkubo, M. Koike, O. Okuno, Y. Oda, JOM-US , 56 (2004), pp. 46-48
[22] F.F. Cardoso, A. Cremasco, R.J. Contieri, E.S.N. Lopes, C.R.M. Afonso, R. Caram, Mater. Des. , 32 (2011), pp. 4608-4613
[23] X.L. Meng, M. Sato, A. Ishida, Philos. Mag. Lett. , 88 (2008), pp. 575-582
[24] V.N. Eremenko, YuI. Buyanov, S.B. Prima, Soviet Powder Metall. Metal Ceramics , 5 (1966), pp. 494-502
[25] K.Y. Zhu, Y.Q. Zhao, H.L. Qu, Z.L. Wu, X.M. Zhao, J. Mater. Sci. , 35 (2000), pp. 5609-5612
[26] M. Koike, Z. Cai, Y. Oda, M. Hattori, H. Fujii, T. Okabe, J. Biomed. Mater. Res. Part B , 73 (2005), pp. 368-374
[27] T. Aoki, I.C.I. Okafor, I. Watanabe, M. Hattori, Y. Oda, T. Okabe, J. Oral Rehabil. , 31 (2004), pp. 1109-1114
[28] C. Ohkubo, Biomaterials , 24 (2003), pp. 3377-3381
[29] Z. Hu, Y. Zhan, J. She, G. Zhang, D. Peng, J. Alloy. Compd. , 485 (2009), pp. 261-263
[30] Z.Y. Suo, K.Q. Qiu, Q.F. Li, Y.L. Ren, Z.Q. Hu, J. Alloy. Compd. , 463 (2008), pp. 564-568
[31] T. Shirai, H. Tsuchiya, T. Shimizu, K. Ohtani, Y. Zen, K. Tomita, J. Biomed. Mater. Res. Part B , 91 (2009), pp. 373-380
[32] E. Zhang, F. Li, H. Wang, J. Liu, C. Wang, M. Li, K. Yang, Mater. Sci. Eng. C , 33 (2013), pp. 4280-4287
[33] L. Ren, Z. Ma, M. Li, Y. Zhang, W. Liu, Z. Liao, K. Yang, J. Mater. Sci. Technol. , 30 (2014), pp. 699-705
[34] G. Lütjering, J.C. Williams, Titanium, (second ed.)Springer, Verlag Berlin Heidelberg (2003)
[35] L. Ren, K. Yang, L. Guo, H.W. Chai, Mater. Sci. Eng. C , 32 (2012), pp. 1204-1209
[36] V.A.A.E. ISO-10993-5, Biological Evaluation of Medical Devices - Part 5: Tests in Vitro for Cytotoxicity: in Vitro Methods, Arlington.
[37] U. Gelius, A.B. Kolpachev, O.V. Kolpacheva, I.Y. Nikiforov, A.A. Chularis, J. Struct. Chem. , 42 (2001), pp. 578-582
[38] M. Kikuchi, Y. Takada, S. Kiyosue, M. Yoda, M. Woldu, Z. Cai, O. Okuno, T. Okabe, Dental Mater. , 19 (2003), pp. 375-381
[39] IPCS, Copper: Environmental Health Criteria 200
International Programme on Chemical Safety, World Health Organization, Geneva (1998)